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| 莨菪发根培养体系的建立 | |
| 引用本文： | 陆倍倍,张磊,开国银,张汉明,丁如贤,陈万生. 莨菪发根培养体系的建立[J]. 中草药, 2005, 36(12): 1864-1868 |
| 作者姓名： | 陆倍倍 张磊 开国银 张汉明 丁如贤 陈万生 |
| 作者单位： | 1. 第二军医大学药学院,生药教研室,上海,200433 2. 上海交通大学植物生物技术研究中心,农业与生物学院复旦-交大-诺丁汉植物生物技术研发中心,生命科学技术学院,上海,200030 3. 第二军医大学长征医院,上海,200003 |
| 基金项目： | 江苏省药用植物生物技术重点实验室开放基金(KJS03080) |
| 摘 要： | 目的建立莨菪Hyoscyamus niger的发根培养体系。方法分别用A4、LBA9402和C58C1三种发根农杆菌菌株转化莨菪子叶受体,获得发根;测定发根的生长曲线和比较不同因素对发根生长量的影响;PCR鉴定转化发根;莨菪发根中生物碱类含量的测定。结果首次利用发根农杆菌A4、LBA9402、C58C1三种菌株成功从莨菪中诱导出发根,并建立了莨菪发根最优的培养体系;经PCR鉴定证明Ri质粒的T-DNA已转化进莨菪的发根中。结论莨菪发根培养体系的建立为利用遗传代谢工程手段调节莨菪次生代谢物含量和进行药用活性成分的工业化生产奠定了基础。 |
| 关 键 词： | 莨菪 发根农杆菌 发根 |
| 文章编号： | 0253-2670（2005）12-1864-05 |
| 收稿时间： | 2005-02-21 |
| 修稿时间： | 2005-02-21 |
Establishment of hairy root culture of Hyoscyamus niger |
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| LU Bei-bei,ZHANG Lei,KAI Guo-yin,ZHANG Han-ming,DING Ru-xian and CHEN Wan-sheng. Establishment of hairy root culture of Hyoscyamus niger[J]. Chinese Traditional and Herbal Drugs, 2005, 36(12): 1864-1868 | |
| Authors: | LU Bei-bei ZHANG Lei KAI Guo-yin ZHANG Han-ming DING Ru-xian CHEN Wan-sheng |
| Affiliation: | 1. Department of Pharmacognosy, School of Pharmacy, The Second Military Medical University, Shanghai 200433, China ; 2. Plant Biotechnology Research Center of Shanghai Jiao Tong University, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology Reaearch and Development Center, School of Life Science and Technology, Shanghai 200030, China; 3. Changzheng Hospital of the Second Military Medical University, Shanghai 200003, China |
| Abstract: | Objective To establish the cultural system for hairy root of Hyoscyamus niger. Methods Hairy root of H. niger was obtained from cotyledon explants after infected with Agrobacterium rhizogenes strains A4, LBA9402, and C58C1, respectively. The growth curves were determined and the effects of different factors on the growth of hairy root were compared. The transformation of Ri T-DNA was examined by PCR. And the contents of alkaloids in hairy root were determined. Results The hairy root of H. niger was successfully induced by using A. rhizogenes strains A4, LBA9402, and C58C1 for the first time and the best system of hairy root culture was established. The transformation of T-DNA from Ri plasmid to the hairy root was confirmed by PCR analysis. Conclusion The establishment of cultural system for hairy root of H. niger lays foundation for regulating the secondary metabolism of H. niger by genetic engineering technique and for the large-scale production of active drug components. |
| Keywords: | Hyoscyamus niger L. Agrobacterium rhizogenes hairy roots |
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