|
|||||
|
|
| 胶体染料试纸条法(DDIA)诊断湄公血吸虫病的研究 | |
| 引用本文： | 朱荫昌,Duong Socheat,梁幼生,Muth Sinuon,何伟,徐明,史伟珠. 胶体染料试纸条法(DDIA)诊断湄公血吸虫病的研究[J]. 中国血吸虫病防治杂志, 2004, 16(3): 161-163 |
| 作者姓名： | 朱荫昌 Duong Socheat 梁幼生 Muth Sinuon 何伟 徐明 史伟珠 |
| 作者单位： | 1. 江苏省寄生虫病防治研究所,无锡,214064 2. 柬埔寨国家寄生虫学、昆虫学和疟疾防治中心 3. 老挝国家实验室和流行病学中心 4. 联合国开发署/世界银行/世界卫生组织热带病研究和培训特别规划署(TDR) |
| 基金项目： | 联合国发展计划署(UNDP)资助项目，亚洲血吸虫病网络资助项目，江苏省卫生厅科研项目 |
| 摘 要： | 目的评价胶体染料试纸条法诊断湄公血吸虫病的应用价值.方法制备以日本血吸虫虫卵可溶性抗原为抗原的胶体染料试纸条试剂盒(DDIA kit).采用该试剂盒检测来自柬埔寨湄公血吸虫病流行区的34例粪检阳性者和170例粪检阴性者,近年来经吡喹酮治疗后不同年份的人群122例,以及114例来自非血吸虫病流行区的健康人;同时对来自老挝湄公血吸虫病流行区的70例粪检阳性者和60例猫后睾吸虫感染者进行检测.对其中部分样本还采用日本血吸虫SEA-ELISA进行对照检测.结果 DDIA试剂盒检测湄公血吸虫病的敏感性为97.1%(33/34)(柬埔寨)和98.6%(69/70)(老挝),与ELISA的检测结果相一致.对非流行区健康人的特异性为100.0%.结论日本血吸虫DDIA试剂盒具有快速、简便、不需任何仪器设备等优点,同样适用于对湄公血吸虫病的现场大规模筛查. |
| 关 键 词： | 胶体染料试纸条法 湄公血吸虫病 免疫诊断 |
| 文章编号： | 1005-6661(2004)03-0161-03 |
| 修稿时间： | 2004-04-05 |
APPLICATION OF DIPSTICK DYE IMMUNOASSAY (DDIA) KIT FOR SCHISTOSOMIASIS MEKONGI DIAGNOSIS IN CAMBODIA AND LAOS |
|
| Duong Socheat,Khanthong Bounlu,Muth Sinuon,Sithat Insisiengmay,Nils Robert Bergquist. APPLICATION OF DIPSTICK DYE IMMUNOASSAY (DDIA) KIT FOR SCHISTOSOMIASIS MEKONGI DIAGNOSIS IN CAMBODIA AND LAOS[J]. Chinese journal of schistosomiasis control, 2004, 16(3): 161-163 | |
| Authors: | Duong Socheat Khanthong Bounlu Muth Sinuon Sithat Insisiengmay Nils Robert Bergquist |
| Abstract: | Objective To evaluate the application value of the dipstick dye immunoassay (DDIA) on schistosomiasis mekongi diagnosis. Methods The dipstick dye immunoassay kit with soluble egg antigen of Schistosoma japonicum was prepared. The people with schistosomiasis mekongi from Cambodia and Laos, as well as the healthy people from a non-endemic area of Cambodia were tested with the DDIA kit. Results The sensitivity and specificity of DDIA were 97.1% (33/34, in Cambodia) ,98.6% (69/70, in Laos), and 100.0%, in Cambodia, respectively. Conclusion DDIA could be used for diagnosis of schistosomiasis mekongi on large scale in the endemic field. |
| Keywords: | Dipstick dye immunoassay (DDIA) Schistosomiasis mekongi Immunodiagnosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |