| JMJD2B通过ERK-MAPK途径影响人结直肠癌细胞的恶性表型 |
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| 引用本文： | 陈丽莎,徐永成,余志金,曾书君,罗 程,程进伟,陈惠新.JMJD2B通过ERK-MAPK途径影响人结直肠癌细胞的恶性表型[J].南京医科大学学报,2015(7):962-967. |
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| 作者姓名： | 陈丽莎 徐永成 余志金 曾书君 罗 程 程进伟 陈惠新 |
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| 作者单位： | 中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000,中山大学附属惠州市中心人民医院消化内科,广东 惠州 516000 |
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| 基金项目： | 广东省自然科学基金面上项目(S201310011923);惠州市科技计划项目(2013Y009) |
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| 摘 要： | 目的:探讨组蛋白去甲基化酶JMJD2B影响人结直肠癌细胞恶性表型所介导的信号通路?方法:以RNA干扰技术靶向沉默人结直肠癌细胞HCT116和SW480中JMJD2B的表达,采用蛋白质印迹法检测人结直肠癌细胞ERK-MAPK信号通路的变化,并分别采用CCK-8?流式细胞分析检测细胞增殖和细胞周期分布?凋亡情况?结果:转染JMJD2B siRNA能特异性抑制JMJD2B的表达并导致ERK2表达下调,其磷酸化水平也降低,肿瘤细胞发生G2/M或G0/G1期阻滞,细胞凋亡比例增加,增殖显著受抑(P<0.05)?结论:抑制JMJD2B可通过阻断ERK-MAPK信号转导而抑制人结直肠癌细胞的恶性表型?
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| 关 键 词： | 结直肠肿瘤 组蛋白去甲基化酶JMJD2B 细胞外信号调节MAP激酶类 细胞增殖 |
| 收稿时间： | 2014/12/7 0:00:00 |
Effects of JMJD2B mediated ERK-MAPK signaling pathway in malignant phenotype of human colorectal cancer cells |
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| Chen Lish,Xu Yongcheng,Yu Zhijin,Zeng Shujun,Luo Cheng,Cheng Jinwei and Chen Huixi.Effects of JMJD2B mediated ERK-MAPK signaling pathway in malignant phenotype of human colorectal cancer cells[J].Acta Universitatis Medicinalis Nanjing,2015(7):962-967. |
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| Authors: | Chen Lish Xu Yongcheng Yu Zhijin Zeng Shujun Luo Cheng Cheng Jinwei and Chen Huixi |
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| Institution: | Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China,Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China,Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China,Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China,Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China,Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China and Department of Gastroenterology, Sun Yat-sen University Affiliated Huizhou Municipal Central Hospital, Huizhou 516000,China |
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| Abstract: | Objective:To investigate the signal pathway through which JMJD2B affected the malignant phenotype of human colorectal cancer cells. Methods: Human colorectal cancer cell lines HCT116 and SW1480 were interfered with siRNAs for silencing JMJD2B expression. The expression of ERK-MAPK signal pathway was detected by Western blotting, and cell proliferation was determined by CCK-8 assay, while cell cycle distribution and apoptosis were assessed by flow cytometry. Results: JMJD2B siRNA effectively and specifically inhibited the expression of JMJDB, which decreased ERK2 and its phosphorylation expression, and subsequently led to cell cycle arrest in G2/M or G0/G1 phase, increasing rates of cell apoptosis and significant cell proliferation inhibition (P < 0.05). Conclusion: Inhibition of histone demethylase JMJD2B restrained the malignant phenotype of human colorectal cancer cells via the inhibition of ERK-MAPK signal pathway transduction. |
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| Keywords: | colorectal tumor histone demethylase JMJD2B extracellular-signal regulated MAP kinases cellular proliferation |
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