乙醇对大鼠血压和去甲肾上腺素加压反应的影响

目的 探讨乙醇对大鼠动脉血压及去甲肾上腺素加压反应的影响及其可能机制.方法40只雄性Wistar大鼠随机分成四组:对照组(10只),其它三组(各10只)分别给予外源性乙醇0.7g·kg~(-1)·d~(-1),1.4g·kg~(-1)·d~(-1),和2.1g·kg~(-1)·d~(-1)共3个月.分别于1月、2月和3月末测尾动脉电压,3月未取出肠系膜血管网行离体灌注,分别给予去甲肾上腺素1.2×10~(-8)mol/L,1.2×10~(-6)mol/L 100ul,记录反应开始时间,反应持续时间及最高灌注压.结果(1)与对照组比较,经乙醇处理大鼠的动脉血压升高,乙醇日摄入量愈多血压升高愈明显分别是:收缩压90.07±2.05mmHg,101.98±2.97mmHg,105.51±6.38mmHg,113.97±8.81mmHg,(与对照组比较P均<0.01;乙醇日摄入量最高组比较最低组P<0.01),舒张压80.11±2.42mmHg,90.34±3.77mmHg,91 03±5.01mmHg,91.67±5.37mmHg(与对照组比较P均<0.01)],3个月末时差异仍存在;(2)各实验组大鼠离体肠系膜动脉对去甲肾上腺素反应开始时间无明显差异(P>0.05);(3)与对照组比较,经乙醇处理大鼠离体肠系膜动脉对去甲肾上腺素反应持续时间延长(P均<0.01),乙醇日摄入量愈多延长愈明显(P<0.01);(4)与对照组比较,经乙醇处理大鼠离体肠系膜动脉对去甲肾上腺素反应的最高灌流压升高(P均<0.01),乙醇日摄入量愈

Effect of alcohol on blood pressure and vascular reactivity to norepinephrine in rats

Objective To observe the action of alcohol on blood pressure and the peripheral vascular response to norepinephrine. Methods Forty male wistar rats were divided into four grops:(l) control group (n = 10), (2)alcohol-treated(0. 7g ?kg-1 ?d-1) group 2(n = 10),(3) alochol-treated(1. 4g ?kg-1 ?d-1) group 3 (n = 10) for 3 months. Their blood pressure were measured after 1 months, 2 months and 3 months. Mesenteric arterial perfusin ex vivo were performed. Norepinephrine (1. 2×108mol/L, 1. 2 ×10-7mol/L and 1. 2×10-6mol/L in 100ul respectively) were administered by bolus injection. The starting time and lasting time to norepinephrine and the highest perfusion pressure for mesenteric arteries were constantly monitored by ploygraph. Results (1)The systolic blood pressure was significantly increased in Wistar rats treated with alcohol than that without treatment (90. 07 ±2. 05mmHg, 101. 98±2. 97mmHg, 105. 51±6. 38mmHg and 113. 97±8. 81mmHg respectively, all P<0. 01) , The higher the alcohol taken, th systolic blood pressure become(113. 97±8. 81mmHg vs 101. 98±2. 97mmHg, P<0. 01). So do their diastolic blood ressure (80. 11±2. 42mmHg,90. 34±3. 77mmHg, 91. 03±5. 01mmHg and 91. 67±5. 37mmHg, 90. 34±3. 77mmHg,91. 03 ± 5.01mmHg and 91. 67±5. 37mmHg respectively, all P<0. 01). (2)A11 rats were not difference in starting time to react to norepinephrine (P>0. 05). (3)The lasting time to react to norepinephrine was significantly increased in Wistar rats treated with alcohel than that without (all P<0. 01). The higher the alcohol taken, the longer the lasting time to react to norepinephrine be-come(P<0. 01). (4)The highest perfusion pressure for mesenteric arteries in rats treated with alcohol showed a significantly increase compared with control group(all P<0. 01). The higher the alcohol taken, the higher the perfusinon pressure become(P< 0.01). Conclusion Alcohol may result in hypertension by influencing peripheral vascular response to pressor agonists.