| 多肿瘤抑制基因对卵巢癌细胞周期的影响 |
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| 引用本文： | 刘淑娟,辛晓燕. 多肿瘤抑制基因对卵巢癌细胞周期的影响[J]. 西北国防医学杂志, 2001, 22(2): 151-153 |
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| 作者姓名： | 刘淑娟 辛晓燕 |
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| 作者单位： | 第四军医大学西京医院妇产科, |
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| 摘 要： | 目的：在以往研究的基础上进一步探讨多肿瘤抑制基因（MTSI）对卵巢癌细胞周期的影响。方法：将卵巢癌细胞（HO8910）和转染空载体的卵巢癌细胞（8910－pcDNA2)作为对照组，与转染MTS1的卵巢癌细胞（8910－p16)同时进行消化收集后经流式细胞仪检测，对比各组间细胞周期的改变。结果：HO－8910细胞经MTS1转染后，其受阻于G1期的细胞增多，同时S期细胞相应减少，而单纯转染空载体并不对细胞周期产生任何影响。结论：MTS1基因可以改变卵巢癌细胞HO－8910的细胞周期，这可能为卵巢癌的基因治疗提供了新的实验依据。
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| 关 键 词： | 卵巢癌 多肿瘤抑制基因 细胞周期 流式细胞术 |
| 文章编号： | 1007-8622(2001)02-0151-03 |
| 修稿时间： | 2000-09-10 |
The effect of multiple tumor suppressor 1(MTS1) on the cell cycle of human ovarian cancer cell |
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| LIU Shu-juan,XIN Xiao-yan. The effect of multiple tumor suppressor 1(MTS1) on the cell cycle of human ovarian cancer cell[J]. Medical Journal of National Defending Forces in Northwest China, 2001, 22(2): 151-153 |
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| Authors: | LIU Shu-juan XIN Xiao-yan |
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| Abstract: | Objective:To investigate furtherly on the e ffect of MTS1 on the cell cycle of human ovarian cancer cell line(HO-8910) base d on the previous studying.Methods:HO-8910 and 8910 -pcDNA3 as the control groups were digested and collected the same as the tr a nsfected HO-8910(8910-p16) did.The samples were analyzed by FCM(Flow cytometry )respectively and their cell cycles were compared.Results:Comparing to the control groups,FCM analysis of the 8910-p16 cells showed that the ratio of S phase cell decreased obviously and the ratio of G1 phase cell increased.There was no difference between the HO-8910 and 8910-pcDNA3 cel ls.Conclusion:MTS1 is able to disturb the cell cycle of HO-8910 and suppress the cell division.This provides a further evidence for gene therapy of MTS1 on human ovarian cancer. |
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| Keywords: | Ovarian cancer Multiple tumor suppresso r 1 Cell cycle Flow cytometry |
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