circ_0001361靶向miR-486-3p对乳腺癌细胞增殖和凋亡的影响

摘 要：［目的］ 探讨circ_0001361靶向miR-486-3p对乳腺癌细胞增殖和凋亡的影响。［方法］ 通过集落形成实验、流式细胞术、CCK-8法评估circ_0001361和miR-486-3p对乳腺癌MDA-MB-231细胞克隆、凋亡以及细胞活力的影响。双荧光素酶检测circ_0001361与miR-486-3p的相互作用。［结果］ 干扰circ_0001361表达显著性促进细胞凋亡（7.16%±0.65% vs 25.27%±2.32%，P<0.001）、上调miR-486-3p表达（0.96±0.06 vs 3.08±0.29，P<0.001）、降低细胞光密度值（0.82±0.07 vs 0.44±0.04，P<0.001）和克隆形成数（84.47±7.23 vs 39.69±3.76，P<0.001）。过表达circ_0001361显著性下调miR-486-3p表达（1.00±0.00 vs 0.45±0.05，P<0.001）、促进细胞凋亡（7.36%±0.66% vs 20.44%±2.17%，P<0.001）、降低细胞细胞光密度值（0.86±0.06 vs 0.51±0.05，P<0.001）和克隆形成数（89.62±7.35 vs 45.81±4.48，P<0.001）。circ_0001361与miR-486-3p直接结合。下调miR-486-3p表达显著性减弱干扰circ_0001361对MDA-MB-231细胞克隆形成数、凋亡率和细胞光密度值的影响（P<0.001）。［结论］ 干扰circ_0001361通过促进miR-486-3p表达来诱导乳腺癌细胞凋亡，并抑制其增殖。

Effect of circ_0001361 Targeting miR-486-3p on Proliferation and Apoptosis of Breast Cancer Cell

Abstract：［Objective］ To investigate the effect of circ_0001361 on the proliferation and apoptosis of breast cancer cells and its relation with miR-486-3p. ［Methods］ The effect of circ_0001361 and miR-486-3p on the cloning， apoptosis and cell viability of breast cancer MDA-MB-231 cells were detected by colony formation assay， flow cytometry and CCK-8 assay， respectively. The interaction between circ_0001361 and miR-486-3p was detected by dual-luciferase reporter assay. ［Results］The circ_0001361 knockdown significantly promoted cell apoptosis（7.16%±0.65% vs 25.27%±2.32%， P<0.001)， up-regulated miR-486-3p expression(0.96±0.06 vs 3.08±0.29， P<0.001)， and decreased the cell proliferation(0.82±0.07 vs 0.44±0.04， P<0.001) and clone numbers(84.47±7.23 vs 39.69±3.76， P<0.001). Overexpression of circ_0001361 significantly down-regulated miR-486-3p expression(1.00±0.00 vs 0.45±0.05， P<0.001). Overexpression of miR-486-3p significantly promoted cell apoptosis(7.36%±0.66% vs 20.44%±2.17%， P<0.001)， decreased cell proliferation(0.86±0.06 vs 0.51±0.05，P<0.001) and clone numbers(89.62±7.35 vs 45.81±4.48，P<0.001). The circ_0001361 directly bound with miR-486-3p. Down-regulating miR-486-3p significantly reduced the effect induced by circ_0001361 interference on the colony formation numbers， apoptosis rate and proliferation of MDA-MB-231 cells(P<0.001). ［Conclusion］ Interference circ_0001361 induces breast cancer cell apoptosis and inhibits cell proliferation through promoting miR-486-3p expression.