| 庆大霉素对豚鼠血管纹Na，K-ATP酶α亚单位异构体表达的影响 |
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| 引用本文： | 钟时勋,刘兆华. 庆大霉素对豚鼠血管纹Na，K-ATP酶α亚单位异构体表达的影响[J]. 听力学及言语疾病杂志, 2000, 8(3): 137-139 |
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| 作者姓名： | 钟时勋 刘兆华 |
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| 作者单位： | 第三军医大学附属大坪医院野战外科研究所耳鼻咽喉-头颈外科重庆 400042 |
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| 摘 要： | 目的 观察正常豚鼠血管纹Ｎａ，Ｋ－ＡＴＰ酶α亚单位异构体的表达及庆大霉素对其表达的影响。方法 用鼠抗大鼠Ｎａ，Ｋ－ＡＴＰ酶α亚单位异构体特异性单克隆抗体，采用免疫组化ＳＰ法观察正常豚鼠血管纹中Ｎａ，Ｋ－ＡＴＰ酶α１异构体的表达及庆大霉素作用后表达的变化。结果 正常豚鼠血管纹Ｎａ，Ｋ－ＡＴＰ酶α１异构体表达呈阳性，而α２和α３异构体表达呈阴性。庆大霉素作用后α１异构体表达显著减弱。结论 庆大霉素显著
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| 关 键 词： | 庆大霉素 Na K－ATP霉 α血管纹 |
| 文章编号： | 1006-7299(2000)03-0137-03 |
| 修稿时间： | 1999-11-17 |
Effect of Gentamicin on the Expression of Subunit Isoforms of Na, K-ATPase in Stria Vascualris of Guinea Pig |
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| Zhong Shixun,Liu Zhaohua. Effect of Gentamicin on the Expression of Subunit Isoforms of Na, K-ATPase in Stria Vascualris of Guinea Pig[J]. Journal of Audiology and Speech Pathology, 2000, 8(3): 137-139 |
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| Authors: | Zhong Shixun Liu Zhaohua |
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| Abstract: | Objective To investigate the expression of asubunit isforms of Na,K-ATPase in stria vascularis of guinea pig, and the effect of gentamicin on it. Methods The expression of a subunit isoforms of Na, K-ATPase in stria vascualris of guinea pig and its change after gentamicin exposure were identified immunocytochemically using the specific mouse antibodies a gainst the α1 ,α2 and α3 subunits of rat Na,K-ATPase. Results All marginal cells,intermediate cells and basal cells of stria vas cualris showed positive expression of α1 isoform and negative expression of α2 and α3 isoforms. The immunostaining of α1 isform was attenuated significantly after gentamicin esposture. Conclusion Gentamicin significantly inhibits the expression of Na, K-ATPase α1 subunit isoform of the stria vascularis, sequentially inhibits the activity of Na,K-ATPase. |
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| Keywords: | Gentamicin Na K-exchanging ATPase Stria vascularis |
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