Association between polymorphisms in the biometabolism genes CYP1A1, GSTM1, GSTT1 and GSTP1 in bladder cancer |
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Authors: | Jo?o Paulo Souto Grando Hellen Kuasne Roberta Losi-Guembarovski Iara Sant’Ana Rodrigues Henrique Mitsu Matsuda Paulo Emílio Fuganti émerson Pereira Gregório Farid Libos Júnior Rodrigo Paes de Menezes Marco Aurélio de Freitas Rodrigues Ilce Mara de Syllos Cólus |
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Institution: | (1) Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, PR, Brazil;(2) Hospital do Cancer de Londrina, Londrina, PR, Brazil;(3) Departamento de Cirurgia, CCS, Universidade Estadual de Londrina, Londrina, PR, Brazil |
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Abstract: | Numerous enzymes, including Cytochrome P450s (phase I) and Glutathione-S-transferases (phase II), are involved in the metabolic activation and detoxification of carcinogens. Epidemiological studies
have consistently demonstrated that bladder cancer is strongly associated with cigarette smoking, and the risk for the development
of this neoplasia may be modified by individual differences in carcinogen-metabolizing genes. We investigated the relationship
between polymorphisms in the CYP1A1, GSTM1, GSTT1, and GSTP1 genes in a case–control study with 100 bladder cancer patients and 100 controls matched for age, gender, race, and smoking
status. The GSTM1, GSTT1, CYP1A1 (A2455→G), and GSTP1 (A313→G) genotypes were determined using a multiplex PCR, an allele specific PCR, and a restriction fragment length polymorphism-PCR
method. The present case–controlled association study did not detect any positive or negative association for the GSTM1 and GSTP1 genes odds ratios (OR) = 1.35; 95% confidence interval (CI) = 0.76–2.41 and OR = 0.75; 95% CI = 0.41–1.38, respectively].
Notably, the genes GSTT1 and CYP1A1 exhibited a statistically significant association with bladder cancer (OR = 1.77; 95% CI = 1.01–3.12 and OR = 1.99; 95% CI = 1.07–3.73).
No differences for GSTM1 and GSTP1 genotype prevalence between the bladder cancer cases and the controls were observed, however, the null genotype for the GSTT1 gene and the A/G and G/G variants of the CYP1A1 gene may contribute to the development of bladder cancer. |
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Keywords: | Bladder cancer Susceptibility genes CYP1A1 GSTM1 GSTT1 GSTP1 |
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