急性髓系及混合型白血病流式细胞术免疫分型检测结果

 目的　研究流式细胞术（FCM）检测在急性髓系白血病（AML）及混合型白血病（MAL）诊断中的意义。方法　采用四色FCM对52例AML和7例MAL患者进行免疫表型检测。结果　52 例AML患者以系列专一表达为主，主要表达 CD13（94.2 %），CD117（90.4 %），cMPO（90.4 %）CD33（86.5 %），CD34（57.7 %），HLA-DR（53.8 %）。7例MAL中髓系／B系（M／B）混合3例，均表达CD13，CD34，CD117，CD10，CD19，cMPO，cCD79a；髓系／T系（M／T）混合2例，均表达CD13，CD117，CD33，CD34，CD5，CD7，cMPO；B系／T系（B／T）混合2例，均表达CD5，CD7，CD10，CD19，CD20，CD34。且与形态学和组织化学诊断具有高符合率。结论　FCM能够提高AML和MAL的确诊率，对该类白血病的明确诊断及分型具有重要的临床指导意义。

Immunophonotype of acute myeloid leukemia and mixed leukemia detected by FCM

Objective　To detect by Flow cytometry (FCM) acute myeloid leukemia. Methods　Four-color staining was used for analysis of immunphonotypes of 52 cases with acute myeloid leukemia and 7 cases of mixed leukaemia. Results　52 cases with series of patients with AML expression, with the main expression CD13 (94.2 %), CD117 (90.4 %), cMPO (90.4 %) CD33 (86.5 %), CD34 (57.7 %), HLA-DR (53.8 %). In 7 cases of mixed cell leukemia 3 cases of myeloid/B (M/B), are expressing CD13, CD34, CD117, CD10, CD19, cMPO, cCD79a. Myeloid/T (M/T) 2 cases with expression of CD13, CD117, CD33, CD34, CD5,CD7, cMPO; B department/T (B/T) 2 cases, with expression of CD5, CD7, CD10, CD19, CD20, CD34. And with the morphological and organizations with high accuracy of chemical diagnosis. Conclusion　FCM can improve the diagnose rate for AML and mixed leukemia. And has played an important clinical significance.