| 天然和氧化极低密度脂蛋白对人脐静脉内皮细胞VCAM-1表达的影响 |
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| 引用本文： | 瞿智玲,杨全宝,阮秋蓉,朱大和. 天然和氧化极低密度脂蛋白对人脐静脉内皮细胞VCAM-1表达的影响[J]. 华中科技大学学报(医学版), 2004, 33(4): 426-429 |
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| 作者姓名： | 瞿智玲 杨全宝 阮秋蓉 朱大和 |
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| 作者单位： | 1. 华中科技大学同济医学院基础医学院病理学系,武汉,430030
2. 湖北省鄂州市中心医院内科,鄂州,436000 |
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| 基金项目： | 国家自然科学基金资助项目 (No 30 30 0 135 ) |
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| 摘 要： | 目的 探讨天然和氧化极低密度脂蛋白(n-VLDL，ox-VLDL)对培养的人脐静脉内皮细胞(HUVEC)表达血管细胞粘附分子1(VCAM-1)的影响。方法 将n-VLDL和ox-VLDL作用于培养的HUVEC，用细胞酶联免疫吸附试验(cell ELISA)和单核细胞粘附试验检测VCAM-1蛋白在HUVEC的表达，用原位分子杂交检测VCAM-1 mRNA的表达。结果 正常培养的HUVEC表达VCAM-1，n-VLDL和ox-VLDL促进HUVEC表达VCAM-1，尤以ox-VLDL作用更强。单核细胞粘附试验显示，n-VLDL和ox-VLDL促进单核细胞向HUVEC粘附。结论 天然和氧化极低密度脂蛋白可能通过增强血管内皮细胞表达VCAM-1而促进血液单核细胞粘附于血管壁，从而在动脉粥样硬化的早期病变中起重要作用。
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| 关 键 词： | 极低密度脂蛋白 脐静脉 内皮细胞 VCAM-1表达 HUVEC 血管细胞粘附分子1 |
| 修稿时间： | 2004-02-02 |
Effect of Native and Oxidized Very Low Density Lipoprotein on the Expression of VCAM-1 in Human Umbilical Vein Endothelial Cells |
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| Qu Zhiling,Yang Quanbao,Ruan Qiurong et al. Effect of Native and Oxidized Very Low Density Lipoprotein on the Expression of VCAM-1 in Human Umbilical Vein Endothelial Cells[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2004, 33(4): 426-429 |
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| Authors: | Qu Zhiling Yang Quanbao Ruan Qiurong et al |
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| Affiliation: | Qu Zhiling1,Yang Quanbao2,Ruan Qiurong1 et al 1 Department of Pathology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2 Department of Internal Medicine,Central Hospital of Ezhou City,Ezhou 436000 |
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| Abstract: | Objective To investigate the effect of native and oxidized very low density lipoprotein (n-VLDL, ox-VLDL) on the expression of VCAM-1 in the cultured human umbilical vein endothelial cells (HUVEC).Methods After the HUVEC were stimulated by n-VLDL and ox-VLDL for 24 h, the expression of VCAM-1 protein in HUVEC was detected by cell enzyme linked immunosorbent assay (cell ELISA) and monocytes adhesion test. The expression of VCAM-1 mRNA was detected by in situ hybridization. Results The cultured HUVEC could express VCAM-1. Both n-VLDL and ox-VLDL could enhance the expression of VCAM-1, especially ox-VLDL. The results of monocyte adhesion test revealed that n-VLDL and ox-VLDL could enhance the adhesion of monocytes to HUVECs. Conclusion n-VLDL and ox-VLDL enhanced the recruitment of monocytes to vascular endothelial cells by stimulating the expression of VCAM-1, thus, they may play an important role in early stage of atherosclerosis. |
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| Keywords: | lipoprotein very low density vascular cell adhesion molecule 1 endothelial cells atherosclerosis |
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