LOX-1在氧化型低密度脂蛋白诱导U937细胞凋亡中的作用和卡托普利的干预作用

目的探讨ox-LDL受体LOX-1在ox-LDL诱导单核/巨噬细胞凋亡中的角色和卡托普利的干预作用。方法应用流式细胞术检测细胞凋亡,采用免疫组织化学技术和Western Blot测定Caspase3、8、9的表达。结果ox-LDL可诱导U937细胞的凋亡峰出现(12.773±1.413),应用卡托普利和LOX-1的阻断剂角叉菜胶、PIA后凋亡峰明显减少,其百分比分别为(1.02±0.166)(4.94±0.47)(2.62±0.656),同时U937细胞在用ox-LDL培养后代表线粒体通路的Cas-pase9,3和代表死亡受体通路caspases8,3表达都增加,应用卡托普利和LOX-1的阻断剂角叉菜胶、PIA后caspase3、8、9的表达均减少。结论ox-LDL是通过与其受体LOX-1结合发挥损伤作用的。卡托普利可以抑制ox-LDL对U937细胞凋亡的诱导作用,从而对单核巨噬细胞起到保护作用。

The Effect of LOX-1 on U937 Cell Apoptosis Induced by Oxidized LDL and the Protective Effect of Captopril

Objective To investigate the effect of LOX-1 on U937 cell apoptosis induced by oxidized LDL and the protective effect of Captopril. Methods U937 cells were preincubated with different LOX-1 inhibitors and captopril for 1 hour,then incubated with ox-LDL 100ug/ml for 24 hours.The change of apoptosis and proteolytic enzymes was observed.The degree of U937 cell apoptosis was determined by flow cytometry.The expression of caspase 3,8 and 9 was detected by immunohistochemistry and Western Blot.Results Incubation U937 cell with ox-LDL can induce U937 cell apoptosis and significant upregulate the expression of caspase 3,8 and 9.Preincubation U937 cell with LOX-1 inhibitors PIA,carrageenan and captopril for 1 hour could significantly suppresse U937 cell apoptosis induced by ox-LDL and restraine the increase of caspase 3,8 and 9(P<0.05).Conclusions LOX-1 played a key role on U937 cell apoptosis induced by ox-LDL.Ox-LDL/LOX-1 interaction on U937 cell can induce apoptosis via death receptor and mitochondria pathway.Captopril can protect U937 cell from ox-LDL induced apoptosis.