| 吉西他滨对人乳腺癌细胞和人胚肾细胞增殖的影响 |
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| 引用本文： | 韦敏,肖亿. 吉西他滨对人乳腺癌细胞和人胚肾细胞增殖的影响[J]. 中国医院药学杂志, 2011, 0(8) |
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| 作者姓名： | 韦敏 肖亿 |
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| 作者单位： | 广西医科大学附属第五医院; |
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| 基金项目： | 广西卫生厅自筹基金课题(编号:Z2010056) |
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| 摘 要： | 目的:观察吉西他滨在体外对人胚肾细胞HEK293和人乳腺癌细胞MCF-7增殖的影响。方法:采用MTT法测定吉西他滨对人胚肾细胞HEK293和人乳腺癌细胞MCF-7增殖的影响,比较两者IC50值,绘制细胞生长曲线;并测定吉西他滨作用后细胞培养上清液中SOD,MDA,LDH值的变化。结果:吉西他滨在体外对人乳腺癌细胞MCF-7的增殖抑制作用较强,同时对人胚肾细胞HEK293的增殖有一定的抑制作用,两者的化疗指数为分别为26.907(24h),15.307(48h),10.264(72h)。结论:吉西他滨对人乳腺癌细胞MCF-7有明显增殖抑制作用,同时可抑制人胚肾细胞HEK293的增殖,对肾脏有一定的毒性。
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| 关 键 词： | 吉西他滨 人胚肾细胞HEK293 人乳腺癌细胞MCF-7 增殖抑制 体外 |
Nephrotoxicity of gemcitabine on human breast cancer cell MCF-7 and humanermbryonic kidney cell 293 invitro |
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| WEI Min,XIAO Yi. Nephrotoxicity of gemcitabine on human breast cancer cell MCF-7 and humanermbryonic kidney cell 293 invitro[J]. Chinese Journal of Hospital Pharmacy, 2011, 0(8) |
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| Authors: | WEI Min XIAO Yi |
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| Affiliation: | WEI Min,XIAO Yi(Department of Medical of the Fifth Affiliated Hospital,Guangxi Liuzhou 545005,China) |
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| Abstract: | OBJECTIVE To demonstrate the antiproliferative effect of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 in vitro.METHODS The inhibiting rate of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 was detected by MTT assay.Their IC50 values for the effect of gemcitabine were compared.The cell growth curve was designed.The contents of SOD,MDA and LDH in cultural supernate were measured by ultraviolet spectrophotometry.RESULTS Gemcitabine signi... |
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| Keywords: | gemcitabine human ermbryonic kidney cell 293 human breast cancer cell MCF-7 antiproliferative in vitro |
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