全氟辛酸和全氟辛烷磺酸经大鼠离体皮肤吸收实验

目的]探讨全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）是否能体外经皮吸收及其吸收特征。方法]采用Franz扩散装置，以10μmol／L的PFOA和PFOS的混合溶液（1：1）作为供体液，用HPLC．MS测定两种化合物的单位面积SD大鼠离体皮肤的累积渗透量，分别计算其稳态渗透速率、渗透滞后时间和渗透系数。结果]各透过时间点受体液中均可检测到PFOA和PFOS；PFOA和PFOS的12h累积渗透量分别为（4．97±1．80）、（1．76±1．06）μg/cm2，稳态渗透速率分别为（0．48±0．19）、（0．16±0．06）μg／cm2·h，渗透滞后时间分别为（0．31±0．18）、（0．50±0．06）h，渗透系数分别为（11．48±4．51）×10^-2、（3．17±1．22）×10^-2cm／h。结论]PFOA和PFOS均能够经皮吸收，前者稳态渗透快于后者，渗透系数大于后者，渗透滞后时间明显小于后者。该研究确定了PFOA和PFOS的体外经皮吸收特征，为暴露量评定和危险性评价提供了有意义的基础研究数据。

In Vitro Experiment on Percutaneous Absorption of Perfluorooctanoate (PFOA) and Perfluorooctane Sulfonate (PFOS)

Objective ] To study the pereutaneous penetration of perfluorooctanoate acid（ PFOA ）and perfluorooctane sulfonate（ PFOS ）as well as its absorption characteristics through excised rat skin in vitro. Methods ] Using finite dose of 10 μmol/L PFOA and PFOS mixed solution as body fluids combined with Franz diffusion cells and HPLC-MS method, the differences of accumulative permeating amounts between PFOA and PFOS were measured, and their steady-state penetration rates, lag time and permeability coefficients were calculated. Results ] PFOA and PFOS could be detected in receptor fluids at each time point, the calculated PFOA and PFOS cumulative permeation amounts at 12 h, steady-state penetration rates, lag time, permeability coefficients were （ 4.97± 1.80 ） and （ 1.76 ± 1.06 ） μg/cm2, （ 0.48 ± 0.19 ） and （ 0.16 ± 0.06 ） μg/cm2 ·h, （ 0.31 ±0.18 ） and （ 0.50 ± 0.06 ）h,（ 11.48 ± 4.51 ） ×l0^-2and（ 3.17 ± 1.22 ） × 10^-2cm/h respectively. Conclusion ] PFOA and PFOS were able to conduct percutaneous absorption, the formers penetrated at a faster and higher rate with significant shorter lag time. The study identified the characteristics of PFOA and PFOS percutaneous absorption, provided basic research data for exposure and risk assessment.