Increase in SNAP-25 immunoreactivity in the mossy fibers following transient forebrain ischemia in the gerbil |
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Authors: | E Martí I Ferrer J Ballabriga J Blasi |
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Institution: | (1) Departament de Biologia Cellular i Anatomia Patològica, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga s/n, E-08907 L’Hospitalet de Llobregat, Spain Tel.: 34-3-402 42 79; Fax: 34-3-402 42 13; e-mail: blasi@bellvitge.bvg.ub.es, ES;(2) Unitat de Neuropatologia, Servei d’Anatomia Patològica, Hospital Prònceps d’Espanya, L’Hospitalet de Llobregat, Spain, ES |
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Abstract: | SNAP-25 (a synaptosomal-associated protein of 25 kDa) has been shown to be involved both in synaptic vesicle exocytosis and
in axonal outgrowth. In the present study, we investigated the changes in SNAP-25 immunoreactivity in the hippocampus of the
Mongolian gerbil (Meriones unguiculatus) at different time points after transient forebrain ischemia insult. In parallel, immunostaining for GAP-43, a protein involved
in axonal outgrowth, and for syntaxin-1 (stx1A and stx1B), another protein implicated in neurotransmitter release, was also
analyzed. The animals were subjected to 2.5 or 5 min of transient forebrain ischemia through bilateral common carotid occlusion,
and examined at different intervals after ischemia. SNAP-25 immunoreactivity was increased in the mossy fiber layer as early
as 2 days after 5 min of ischemia. Increased SNAP-25 immunoreactivity in mossy fibers was also detected at days 4 and 7 after
ischemia. On day 15, SNAP-25 staining was similar to that observed in control non-ischemic animals. In contrast, no changes
in GAP-43 and syntaxin-1 immunoreactivity were observed in the mossy fiber layer following 5 min of ischemia. No modifications
in SNAP-25, syntaxin-1 or GAP-43 immunoreactivity were observed following 2.5 min of ischemia, the longest period for which
no neuronal damage is observed. These results provide evidence of a specific involvement of SNAP-25 in the reactive changes
associated with transient forebrain ischemia.
Received: 30 June 1997 / Revised, accepted: 26 September 1997 |
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Keywords: | Ischemia gerbil hippocampus SNAP-25 Delayed cell death |
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