| 检测CYP2A6基因多态性的PCR技术的基因型方法 |
| |
| 引用本文： | 胡春松,朱文玲,程晓曙,苏海,罗伟,洪均言.检测CYP2A6基因多态性的PCR技术的基因型方法[J].中国药理学与毒理学杂志,2004,18(3):233-236. |
| |
| 作者姓名： | 胡春松 朱文玲 程晓曙 苏海 罗伟 洪均言 |
| |
| 作者单位： | 1. 江西医学院心血管病研究所,江西,南昌,330006
2. 中国医学科学院北京协和医院心内科,北京,100730 |
| |
| 基金项目： | 国家留学基金委留学基金 |
| |
| 摘 要： | 综述检测CYP2A6基因多态性的PCR技术的基因型方法。CYP2A6基因多态性存在于白种人、东方人及非洲裔美国人群中 ,包括CYP2A6 1至CYP2A6 12 ,总共 12个变异等位基因。本文列举了检测CYP2A6基因型的不同的DNA来源、引物和限制性内切酶。已开发PCR技术结合诊断性限制性内切酶分析 (RFLP)如两步PCR和一步PCR RFLP方法检测CYP2A6基因型。此外 ,尚有PCR SSCP +RFLP及直接DNA测序方法。
|
| 关 键 词： | 基因 CYP2A6 基因多态性 PCR 基因型 |
| 收稿时间： | 2003-5-20 |
PCR-based genotyping methods for genetic polymorphism of CYP2A6 gene |
| |
| HU Chun-Song, ZHU Wen-Ling, CHENG Xiao-Shu, SU Hai, LUO Wei, HONG Jun-Yan.PCR-based genotyping methods for genetic polymorphism of CYP2A6 gene[J].Chinese Journal of Pharmacology and Toxicology,2004,18(3):233-236. |
| |
| Authors: | HU Chun-Song ZHU Wen-Ling CHENG Xiao-Shu SU Hai LUO Wei HONG Jun-Yan |
| |
| Institution: | (1. Institute of Cardiovascular Disease, Jiangxi Medical College, Nanchang 330006, China; 2. Department of Cardiology, Peking Union Medical College Hosp ital, Chinese Academy of Medical Sciences, Beijing 100730, China) |
| |
| Abstract: | To review PCR based genotyping methods for genetic polymorphism of CYP2A6 gene. Genetic polymorphism of CYP2A6 has been found in Caucasians, Oriental and African American population, which includes from CYP2A6*1 to CYP2A6*12, totally 12 variant alleles. In this article, different DNA source, primers and restriction enzymes for CYP2A6 genotypes were reviewed. Protocols for identification of CYP2A6 genotypes with PCR based techniques combined with diagnostic restriction enzyme digestion such as two step and one step PCR RFLP methods have been developed. Besides, methods of PCR SSCP combined with RFLP and direct DNA sequencing are also introduced. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药理学与毒理学杂志》浏览原始摘要信息 |
| 点击此处可从《中国药理学与毒理学杂志》下载免费的PDF全文 |
|
