鼻咽癌患者治疗中CD34和T细胞亚群检测的意义

[目的]探讨鼻咽癌患者治疗过程中T细胞亚群、CD34检测的临床意义.[方法]应用流式细胞仪测定67例鼻咽癌患者治疗前、治疗4周以及治疗后1个月T细胞亚群、CD34和常规检查血液学情况,20例健康人群为对照组.[结果]血液学常规显示鼻咽癌患者白细胞、血红蛋白、血小板以及尿酸在治疗过程中与对照组比较均无显著性差异(P＞0.05).治疗前CD8+和CD4+/CD8+显著性低于正常组(P＜0.05).患者放疗和同期放化疗期间CD34明显下降,CD3+、CD4+、CD8+、CD4+/CD8+升高.鼻咽癌组治疗后1个月CD8+、CD4+/CD8+显著性高于对照组(P＜0.05).鼻咽癌同期放化疗患者CD34治疗4周和治疗后1个月均显著低于单纯放疗组(P＜0.05),且治疗后1个月CD3+、CD4+显著高于单纯放疗组(P＜0.05).[结论]T细胞亚群变化可评估鼻咽癌患者治疗过程中的免疫功能状态,CD34可用于观察放化疗对骨髓功能影响.

The Value of Detecting CD34 and T Cell Subsets in Patients with Nasopharyngeal Carcinoma During Treatment

[Purpose] To investigate the clinical value of detecting T cell subsets and CD34 during treatment in patients with nasopharyngeal carcinoma(NPC).[Methods] T cell subsets and CD34 of 67 cases with NPC before treatment,were enrolled 4 weeks and 1 month after treatment were detected by flow cytometry.Twenty healthies as controls.[Results] Routine blood test showed that the level of leucocyte,hemoglobin,bloodplatelet and uric acid had no significant difference between NPC group and healthy control group(P>0.05).CD8+ and CD4+/CD8+ in NPC group were significantly higher than those in healthy control group before treatment(P<0.05).CD34 declined sharply during radiotherapy or concurrent radio chemotherapy,but CD3 + and CD4 +,CD8 +,CD4+/CD8+ were elevated.CD8+ and CD4+/CD8+ in NPC group were significantly higher than those in healthy control group at one month after treatment(P<0.05).CD34 in NPC with concurrent radiochemotherapy group was significantly lower than in that NPC with radiotherapy at 4 weeks and one month after treatment(P<0.05).But CD3+ and CD4+ in concurrent radiochemotherapy group were significantly lower than those in radiotherapy group at one month after treatment(P< 0.05).[Conclusion] The changes of T cell subsets are useful for evaluating the status of immunity in NPC.CD34 can be used for surveillance of the impact of radiotherapy and chemotherapy on the bone marrow.