Anticoagulant activity of ilexoside D,a triterpenoid saponin fromIlex pubescens

The anti-coagulant activity of ilexoside D isolated from the roots ofIlex pubescens Hook. et Arn. was investigated inin vivo andin vitro models of blood coagulation in rats. On oral administration, ilexoside D prolonged the bleeding time and the whole blood recalcified clotting time, but not the plasma recalcified clotting time.In vitro, ilexoside D did not affect the recalcified clotting times of whole blood, platelet-rich plasma (PRP), and platelet-poor plasma (PPP) while in the presence of tissue factor the compound prolonged the reduced prothrombin times of whole blood, PRP and PPP in the dose-dependent manner. These results indicate that ilexoside D has the anti-tissue factor activity as well as the antithrombotic activity.