透明质酸支架构建工程化肝组织小块的初步研究

目的探索肝窦血管内皮细胞和肝细胞与透明质酸支架复合后构建工程化肝组织小块的可行性。方法分离小鼠肝细胞和肝窦血管内皮细胞，分别种植于透明质酸海绵状支架上培养，形成支架材料-细胞复合体，植于小鼠肝包膜下肝组织表面。2周后将支架取出观察植入效果。结果分离获得的肝细胞和肝窦血管内皮细胞活力较高。构建的支架-细胞复合物植入体内后与宿主肝组织紧密融合，可见移植物内有微血管形成，血管周围肝细胞聚集生长，结构类似正常肝组织。结论利用透明质酸支架复合肝窦血管内皮细胞和肝细胞构建工程化肝组织小块的方法可行。

Rudimentary construction of liver histioids by hyaluronic acid scaffold

ObjectiveTo study the possibility of the liver histioid construction through co-culturing hepatocytes and sinusoidal vessel endothelial cells (SVECs) on hyaluronic acid(HA) scaffold.MethodsHepatocytes and SVECs of a mouse were isolated respectively, and then were cultured successively in HA scaffold. The scaffold-cell complexes formed later were implanted onto the surfaces of liver in the mouse. After 2 weeks, the implants were taken out for HE staining and compatibility evaluation.ResultsThe isolated hepatocytes and SVECs were vigorous. The implants inosculated well with liver tissue of the host with visible growth of blood vessels in the implants. The hepatocyte aggregation grown around the vessels, the liver-like tissue, were observed.ConclusionIt is feasible to construct liver histioid through co-culturing hepatocytes and sinusoidal vessel endothelial cells on hyaluronic acid scaffold.