毛细管电泳法测定矮地茶中岩白菜素的含量

目的：建立矮地茶药材中岩白菜素的含量测定方法。方法：矮地茶药材粉碎后过筛,取粉末用甲醇超声提取,未涂渍熔融石英毛细管（75μm×48.5 cm,有效长度40 cm）,以25 mmol·L^-1硼砂水溶液为缓冲液,运行电压15 k V,温度25℃,内标为肉桂酸,检测波长为240 nm,运行时间10 min。结果：在此条件下岩白菜素与内标及其他成分能达到基线分离,岩白菜素在3.356-167.800μg·ml^-1范围内呈良好的线性关系Y=0.034 9X＋0.060 6,r=0.997 9,最低检测限为0.839μg·ml^-1（S/N=3）,最低定量限为3.356μg·ml^-1（S/N=10）,平均加样回收率为99.6%（RSD=3.0%,n=6）,低,中,高浓度日内、日间精密度RSD均小于5%（n=3）。结论：此方法可用于矮地茶药材中岩白菜素的含量测定,具有简便快速、准确可靠、试剂消耗量少的优点。

Determination of Bergeni in Herba Ardisiae Japonicae by Capilary Zone Electrophoresis

Objective： To establish a method for the determination of bergeni in Herba Ardisiae Japonicae. Methods： Herba Ardisiae Japonicae was ultrasonic extracted by methanol after crushed and sieved. An untreated fused-silica capillary（ 75 μm ×48. 5 cm,40 cm effective length） was used,the running buffer was 25 mmol ·L^-1 borax in water,the voltage was 15 k V,the temperature was 25℃,the internal standard was cinnamic acid,the ultraviolet wavelength was set at 240 nm,and the running time was 10 minute. Results： Bergeni,the internal standard and the impurities were separated by baseline under the above conditions.Bergeni was linear within the range of 3. 356-167. 800 μg·ml^-1with the linear equation of Y = 0. 034 9X ＋ 0. 060 6（ r = 0. 997 9）,the limit of determination was 0. 839 μg·ml^-1（ S/N = 3）,the limit of quantitation was 3. 356 μg·ml^-1（ S/N = 10）,the average recovery was 99. 6%（ RSD = 3. 1%,n = 6）,and RSDs of intra and inter-day were lower than 5%（ n = 3）. Conclusion： The method has the advantages of convenience,fastness,accuracy,and low sample and reagent consumption,which can be used to determine bergeni in Herba Ardisiae Japonicae.