HPLC法测定复方氢溴酸右美沙芬糖浆中2组分及防腐剂的含量

目的：建立HPLC法测定复方氢溴酸右美沙芬糖浆中2组分及防腐剂的含量。方法：采用Agilent Zobax SB-C18（250mm×4.6 mm,5μm）柱;以甲烷磺酸溶液（取甲烷磺酸4.8 g,加水750 ml,加三乙胺10 ml,用磷酸调节p H值至3.5）-乙腈（75∶25）为流动相;检测波长为280 nm;柱温为30℃;流速为1.0 ml·min^-1;进样量为20μl。结果：愈创甘油醚在102-1 025μg·ml^-1、氢溴酸右美沙芬在15-619μg·ml^-1、苯甲酸在10-407μg·ml^-1范围内,线性关系良好;平均回收率分别为100.0%（RSD=0.35%）、100.1%（RSD=0.77%）、100.8%（RSD=0.49%）。结论：本法简便、快速、准确,可用于复方氢溴酸右美沙芬糖浆的质量控制。

Determination of Two Components and Preservative in Compound Dextromethorphan Hydrobromide Syrups by HPLC

Objective： To establish an HPLC method for the determination of two components and the preservative in compound dextromethorphan hydrobromide syrups. Methods： An Agilent Zobax SB-C18column（ 250 mm × 4. 6 mm,5 μm） was used with methanesulfonic acid solution（ adding 4. 8g methanesulfonic acid and 10 ml triethylamine into 750 ml water,and adjusting the p H value to3. 5 by phosphoric acid）-acetonitrile（ 75∶ 25） as the mobile phase at the flow rate of 1. 0 ml·min^-1 and 280 nm as the detection wavelength. Results： The calibration curve was linear within the range of 102-1 025μg·ml^-1 for guaifenesin,15-619 μg ·ml^-1 for dextromethorphan hydrobromide and 10-407 μg·ml^-1 for benzoic acid. The average recovery of guaifenesin,dextromethorphan hydrobromide and benzoic acid was 100. 0%（ RSD = 0. 35%）,100. 1%（ RSD = 0. 77%） and 100. 8%（ RSD = 0. 49%）,respectively. Conclusion：The method is simple,rapid and accurate,and suitable for the quality assessment of compound dextromethorphan hydrobromide syrups.