UPLC双波长切换法测定银黄连口服液中绿原酸、黄芩苷、连翘苷的含量

目的：建立UPLC双波长切换法同时测定银黄连口服液中绿原酸、黄芩苷、连翘苷三个组分含量的方法。方法：采用Agilent Eclipse Plus-C18色谱柱（100 mm×2.1 mm,1.8μm）,流动相为乙腈-0.4%磷酸溶液,梯度洗脱,流速：0.2 ml·min^-1,波长切换,0-5 min在324 nm波长测定绿原酸含量,5-12 min在277 nm波长测定黄芩苷、连翘苷的含量。结果：绿原酸、黄芩苷、连翘苷分别在浓度39.4-355.0μg·ml^-1（r=0.999 7）、90.0-810.0μg·ml^-1（r=0.999 9）、9.4-84.6μg·ml^-1（r=0.999 5）范围内线性关系良好,平均加样回收率分别为99.44%、98.82%、98.64%。结论：该法简便,可靠,准确,可作为测定银黄连口服液中绿原酸、黄芩苷、连翘苷含量的方法。

Determination of Chlorogenic Acid, Baicalin and Phillyrin in Yinhuanglian Oral Liquids by UPLC with Double wavelength Switch

Objective： To establish a UPLC method for the content determination of chlorogenic acid,baicalin and phillyrin in Yinhuanglian oral liquids. Methods： An Agilent Eclipse Plus C18column（ 100 mm × 2. 1mm,1. 8 μm） was used,the mobile phase was acetonitrile-0. 4% phosphoric acid with gradient elution,and the flow rate was 0. 2 ml·min^-1. The detection wavelength was set at324 nm in the first 5 min for chlorogenic acid,and then changed to 277 nm for baicalin and phillyrin between 5 and 12 min. Results：The linear calibration curve of chlorogenic acid,baicalin and phillyrin was within the range of 39. 4-355. 0 μg·ml^-1（ r = 0. 999 7）,90. 0-810. 0 μg·ml^-1（ r = 0. 999 9） and 9. 4-84. 6 μg·ml^-1（ r = 0. 999 5）,respectively. The average recovery was 99. 44%,98. 82% and 98. 64%,respectively. Conclusion： The method is simple,reliable and accurate in the content determination of chlorogenic acid,baicalin and phillyrin in Yinhuanglian oral liquids.