腰段连续硬膜外阻滞对大鼠妊娠期高血压疾病防治作用的研究

目的:观察连续硬膜外阻滞对大鼠妊娠期高血压疾病的防治作用。方法:Wistar大鼠40只复制妊娠期高血压疾病模型,分为4组,于妊娠14d开始,A组皮下注射生理盐水;B组皮下注射左硝基精氨酸甲酯(L-NAME);C组硬膜外腔置管,但不给药;D组硬膜外腔注入布比卡因。监测妊娠13,20d的体质量、食量、尿量、入水量;从妊娠的13d起,应用鼠尾动脉仪每日监测血压;以磺柳酸法检测孕鼠第13,20日尿蛋白浓度;硝酸还原酶法检测子宫、胎盘组织的一氧化氮(NO)含量。结果:孕20dB、C组与A、D组比较体质量增加(P<0.01),食量下降(P<0.01),入水量无差别(P>0.05),尿量下降(P<0.01);孕20dB、C组与A、D组比较收缩压、心率均显著增高(P<0.01),A组与D组比较心率显著增快(P<0.01);孕20dB、C、D组与A组比较尿蛋白含量明显增加(P<0.01),D组与B、C组比较明显减少(P<0.01);孕20dB、C组与A、D组比较存活率明显下降(P<0.01);孕20dA、D组与B、C组比较NO浓度明显增加(P<0.01)。结论:腰段连续硬膜外阻滞可能对大鼠妊娠期高血压疾病具有防治作用。

Preventive and therapeutic effects of continuous lumbar epidural block in rats with hypertensive disorder complicating pregnancy

Objective:To study the preventive and therapeutic effects of continuous lumbar epidural block in rats with hypertensive disorder complicating pregnancy. Methods:The rat model of hypertensive disorder complicating pregnancy was established according to Olson's method in 40 Wistar rats. The rats were injected with normal saline subcutaneously in group A,L-NAME subcutaneously in group B,and Bupivacaine epidurally in group D. The rats in group C received no drug. The body weight,food intake,urine volume,and water intake were recorded on days 13 and 20 of gestation. The blood pressure was monitored with tail-cuff method from day 13 of the gestation. The urine protein content and the levels of nitric oxide in the uterus and placenta on days 13 and 20 of gestation were determined by sulfosalicylic acid test and nitric acid reductase method,respectively. Results:On day 20 of gestation,compared with groups A and D,the body weight increased and the food intake and urine volume decreased in groups B and C (all P < 0.01),but no significant difference in water intake was found. The systolic blood pressure and heart rate in groups B and C were significantly higher than those in groups A and D on day 20 of gestation,and the heart rate in group A was significantly higher than that in group D (all P < 0.01). The urine protein content in groups B,C,and D was significantly higher than that in group A on day 20 of gestation,and in groups B and C it was significantly higher than that in group D (all P < 0.01). Compared with groups A and D,the survival rate and the level of nitric oxide significantly decreased in groups B and C (P < 0.01). Conclusion:The continuous lumbar epidural block may have preventive and therapeutic effects in rats with hypertensive disorder complicating pregnancy.