电针足三里穴对依托咪酯麻醉大鼠急性应激反应的影响

目的探讨电针足三里穴对依托咪酯麻醉大鼠应激反应的影响。方法选择雄性SD大鼠60只,体重280~310g,随机分为六组:空白对照组(C组)、模型组(M组)、依托咪酯60mg/kg组(E1组)、依托咪酯30 mg/kg组(E2组)、依托咪酯60 mg/kg联合电针组(EA1组)和依托咪酯30mg/kg复合电针组(EA2组),每组10只。C组大鼠不进行任何麻醉手术操作,其余五组均建立低位尾干切断模型。建模后,E1组和EA1组大鼠腹腔注射依托咪酯60mg/kg,E2组和EA2组大鼠腹腔注射依托咪酯30mg/kg。EA1组和EA2组大鼠于麻醉状态行双侧足三里穴治疗30min(频率2/100Hz,疏密波,电流强度1mA)。采用ELISA法检测血清ACTH和Cor的浓度,Western blot法检测下丘脑室旁核组织c-fos蛋白的含量。结果 M组ACTH、Cor浓度和c-fos蛋白含量明显高于C组(P0.05);E1组、E2组、EA1组、EA2组ACTH、Cor浓度和c-fos蛋白含量明显低于M组(P0.05);E2组、EA1组ACTH、Cor浓度和c-fos蛋白含量明显高于E1组(P0.05);EA2组ACTH浓度和c-fos蛋白含量明显低于E2组(P0.05)。结论电针大鼠足三里穴可双向调节依托咪酯对急性应激反应的影响,其机制可能与其调节下丘脑c-fos蛋白水平有关。

Effect of electroacupuncture at tsusanli on regulation of stress response under etomidate anesthesia in rats

Objective To explore the effect of electroacupuncture (EA) at tusanli (ST36) on regulation of stress response under different doses of etomidate anesthesia in rats. Methods Sixty male Sprague-Dawley rats, weighing 280-310 g, were randomly divided into control group (group C), model group (group M), etomidate 60 mg/kg group (group E1), etomidate 30 mg/kg group (group E2), etomidate 60 mg/kg combined with EA group (group EA1) and etomidate 30 mg/kg combined with EA group (group EA2), n=10 in each group. All groups received inferior caudal trunk transection at the level between sacral spinal nerve 3 and 4 (S3, S4) to prepare acute stress response model except group C. Group M received no others treatment. The rats in group E1, group EA1, group E2 and group EA2 were intraperitoneally injected with 60, 60, 30 and 30 mg/kg etomidate, respectively. Group EA1 and group EA2 received EA ST36. The points were stimulated at a frequency of 2/100 Hz with 1 mA output and a dilatational wave, which lasted for 30 min. ACTH and Cor levels were measured by ELISA. The c fos protein expression in hypothalamic tissue was examined by Western blot. Results Compared with group C, ACTH and Cor levels, and hypothalamic expression of c-fos protein in group M were significantly increased (P<0.05). Compared with group M, serum ACTH and Cor levels, and hypothalamic expression of c-fos protein in groups E1, E2, EA1 and EA2 were significantly decreased (P<0.05). Compared with group E1, serum ACTH and Cor levels, and hypothalamic expression of c-fos protein were significantly higher in groups E2 and EA1 (P<0.05). Compared with group E2, serum ACTH level and hypothalamic expression of c-fos protein were significantly lower in group EA2 (P<0.05). Conclusion EA at ST36 regulating stress response under etomidate anesthesia in rats is effective and two-way, and the mechanism may be due to the release of neurotransmitters induced c-fos protein in hypothalamus.