CXCL12/CXCR4在绒毛外滋养细胞中的表达及其意义

目的:研究趋化因子CXCL12及其受体CXCR4在绒毛外滋养细胞(extravillous trophoblasts,EVTs)中的表达及其在妊娠中的作用。方法:取妊娠早期绒毛,培养绒毛外滋养细胞,用免疫细胞化学技术检测其中CXCR4与CXCL12的表达;并通过体外侵袭实验,分析绒毛外滋养细胞在CXCL12作用下的侵袭能力。结果:在EVTs的胞膜和胞浆中均检测到CXCL12和CXCR4的表达;EVTs在CXCL12作用下的侵袭能力在一定范围内与CXCL12浓度呈正相关(r=0.79,P<0.01),当CXCL12浓度达到10ng/ml时最强,达到100ng/ml时则开始下降。最大趋化指数为1.71±0.14。结论:CXCL12/CXCR4受体配体系统参与绒毛外滋养细胞的侵蚀,在妊娠过程中有重要意义。

Expression and significance of CXCR4 and CXCL12 in extra-villous trophoblasts

Objective:To investigate the expression of CL12 and its receptor CR4 in extra-villous trophoblasts (EVTs) so as to find the role in the gestation.Methods:Highly purified EVTs were detected for CR4 and CL12 in vitro by immunocytochemistry.Invasive ability of EVTs under the effect of CL12 was examined quantitatiely by invasive assay in vitro.Results:CL12 and CR4 were found expressed in EVTs.In a certain coverage,EVTs exhibited invasive activity which has positive correlation with the concentration of CL12(r=0.79,P<0.01),the invasive ability of EVTs were the highest when the concentration of CL12 reaches 10ng/ml,and began to decrease when the the concentration is 100ng/ml.The maximum CI(chemo tactic index) was 1.71±0.14.Conclusion:The interaction between CR4 and CL12 contributes to invasion of EVTs which play an important role in pregnancy.