| SPE-HPLC法测定枸杞中甜菜碱的含量 |
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| 引用本文： | 谷大建,李旭.SPE-HPLC法测定枸杞中甜菜碱的含量[J].齐鲁药事,2007,26(4):213-214. |
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| 作者姓名： | 谷大建 李旭 |
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| 作者单位： | 山东省千佛山医院,山东,济南,250014 |
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| 摘 要： | 目的建立测定枸杞中主要成分甜菜碱含量的方法。方法采用Oasis HLB固相萃取小柱对样品进行纯化,C18色谱柱(5μm,250mm×4.6mm),以50mM KH2PO4(pH4.45)和0.3%辛烷磺酸钠为流动相,流速为0.7mL·min-1,检测波长200nm。结果甜菜碱进样量在0.01~0.32μg的范围内与峰面积呈良好线性,r=0.9998。结论该法操作简便,结果准确。
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| 关 键 词： | 甜菜碱 固相萃取 高效液相色谱法 |
| 文章编号： | 1672-7738(2007)04-0213-02 |
Determination of Glycine betaine in leaf of Lycium Chinese Mill. by SPE-HPLC |
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| GU Da-jian,LI Xu.Determination of Glycine betaine in leaf of Lycium Chinese Mill. by SPE-HPLC[J].qilu pharmaceutical affairs,2007,26(4):213-214. |
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| Authors: | GU Da-jian LI Xu |
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| Abstract: | OBJECTIVE To establish a method for determination of Glycine betaine in the leaf of Lycium Chinese Mill. by SPE-HPLC.METHODS Seperation was performed on a stainless steel column(5μm,250mm×4.6mm),eluted with 50mM KH2PO4(pH4.45) containing ion-pair agent 0.3% Sodium-1-Octansulfonate at a flow rate of 0.7mL·min-1.Detection was performed by UV absorbance at 200nm.RESULTS The calibration curve of Glycine betaine was liner in the range of 0.01~0.32μg (r=0.9996).CONCLUSION This method was simple,accurate and reliable. |
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| Keywords: | Glycine betaine SPE HPLC |
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