铁皮石斛多糖提取工艺及脱蛋白方法研究

目的:考察铁皮石斛多糖最佳提取工艺及脱蛋白方法。方法:采用单因素考察和正交试验研究铁皮石斛多糖的最佳提取工艺,并比较盐酸法和三氯乙酸法脱蛋白工艺。结果:铁皮石斛多糖的最佳提取条件为:温度70℃、料液比1∶40、提取时间4 h、提取次数1次,各因素的影响顺序为:温度>时间>提取次数>料液比,盐酸法在p H=3时蛋白脱除率效果最佳。结论:优化的提取工艺稳定可行,铁皮石斛粗多糖的得率可以达到4.67%,盐酸法p H=3时脱蛋白,多糖含量高,更适合于实际生产。

Comparative Study on Extraction of Crude Polysaccharides from Dendrobium Candidum and Removing Protein Procedure

Objective： To study the best extraction condition of crude polysaccharides from Dendrobium Candidum and methods for deproteinization. Method： By combining single- factor and orthogonal experiments to explore the best extraction technology of crude polysaccharides from Dendrobium Candidum,we compared the deproteinization methods of hydrochloric acid and trichloroacetic acid. Results： The results showed that the best extraction condition for crude polysaccharides was as follows. The extraction temperature was 70 ℃. The ratio of solid to liquid was 1 ∶ 40 and the time was 4 h with once extraction. Of the four conditions,temperature had the greatest influence on crude polysaccharides extraction.The best method of protein removal rate was hydrochloric acid method at p H = 3. Conclusion： The optimized extraction process was stable while the yield of crude polysaccharides can reach 4. 67%. When p H = 3,via hydrochloric acid method,the content of polysaccharides can be the highest,which is more suitable for actual production.