基于顶空气相色谱法测定的重楼总皂苷中有机溶剂残留质量控制研究

目的:建立重楼总皂苷中正己烷、苯、甲苯、间二甲苯、对二甲苯、邻二甲苯、苯乙烯和1,2-二乙基苯等共8种有机溶剂残留的测定方法。方法:将重楼总皂苷溶解于25%N,N-二甲基甲酰中,应用顶空气相色谱法,采用石英毛细管柱和氢离子火焰检测器,以高纯氮气为载气,测定重楼总皂苷中8种有机溶剂的残留量。结果:该法可将8种有机残留完全分离,且线性关系良好,r=0.9987~0.9998,精密度RSD均小于5.0%,平均加样回收率为95.24%~98.57%。结论:此法简便、准确、可靠,可用于大孔树脂制备的重楼总皂苷中有机溶剂残的质量控制。

Quality Control over Organic Residues of Rhizoma Paridis Total Saponins Based on Capillary Gas Chromatography with Headspace Sampling

Objective： To develop a method for determining organic residues（ including hexane,benzene,toluene,xylene,p-xylene,o-xylene,styrene and 1,2-diethyl benzene） in Rhizoma Paridis total saponins. Methods： Rhizoma Paridis total saponins were dissolved in 25% of N,N-dimethyl formyl. The organic residues were analyzed by capillary gas chromatography with headspace sampling. Results： The organic residues can be completely separated with this method and good linear relationship of 0. 9987 ~ 0. 9998（ r）. The precision RSD was less than 5. 0%. The recoveries were in the range of 95. 24% ~ 95. 24%. Conclusion： The method is simple,accurate,replicable and can be used for the quality control of organic residues and Rhizoma Paridis total saponins.