Evaluation of mixed chimaerism and origin of bone marrow derived fibroblastoid cells after allogeneic bone marrow transplantation |
| |
| Authors: | Junji Tanaka&dagger ,Masaharu Kasai,Masahiro Imamura&dagger ,Nobuo Masauzi&dagger ,Hiroko Ohizumi&dagger ,Atsushi Matsuura&dagger ,Ken Morii&dagger ,Yoshio Kiyama&dagger ,Tohru Naohara&dagger ,Masao Saitoh&dagger ,Toshio Higa&dagger ,Keisuke Sakurada,Tamotsu Miyazaki |
| |
| Affiliation: | Third Department of Internal Medicine, Hokkaido University, School of Medicine, Kita-ku, Sapporo;Department of Internal Medicine, Sapporo Hokuyu Hospital, Higashi Sapporo, Shiroishi-ku, Sapporo, Japan |
| |
| Abstract: | Summary We assessed the origin of bone marrow derived fibroblastoid cells (BMF) in long-term cultures of 13 samples obtained from nine patients after allo-BMT by polymerase chain reaction (PCR) amplification of MCT118, one of the variable number of tandem repeats regions (VNTR). BMF showed a complete recipient pattern in nine samples obtained from seven patients; however, a recipient-predominant mixed chimaeric pattern was detected in BMF from four patients. Also, two of the four patients died with bone marrow hypoplasia. These data suggest that mixed chimaeric pattern of BMF may be correlated with bone marrow hypoplasia. |
| |
| Keywords: | allogeneic BMT fibroblastoid cells PCR longterm culture |
|
|
