组织蛋白酶B-RNAi-慢病毒对体外类毛细血管形成的抑制

目的 探讨组织蛋白酶β-RNAi-慢病毒CathepsinB-RNAi-Lentivirus（CTSB-RNAi -LV）对体外类毛细血管形成的抑制作用。方法 实验分为对照组和RNAi慢病毒组，采用免疫组织化学和构建体外类毛细血管模型的方法，检测CTSB-RNAi-LV转染小鼠脑微血管内皮细胞(mouse brain micvascular endothelial cells，bEND.3)后对Cathepsin B和类毛细血管形成的影响。结果 免疫组织化学结果显示，bEND.3内Cathepsin B的表达呈棕褐色，主要位于细胞核周围, 各组间Cathepsin B的表达以RNAi慢病毒组最少；bEND.3和培养基总蛋白测定与体外类毛细血管形成实验表明：RNAi慢病毒组总蛋白的含量和体外类毛细血管的形成区域明显低于其余两组，其组间比较有统计学意义（P＜0.05）。结论 CTSB-RNAi- LV能抑制bEND.3内Cathepsin B的表达和体外类毛细血管区域的形成。CTSB-RNAi- LV可靶向抑制体外类毛细血管的形成。

Cathepsin B-RNAi-lentivirusd inhibitis capillary-like tube formation in vitro

Objective To study inhibition of capillary-like formation by Cathepsin B-RNAi-Lentivirus (CTSB-RNAi-LV) EM>in vitro/EM>. Methods The control group and RNAi lentiviral group, and negative control group were tested in this study. Immunohistochemistry method and building capillary-like formation model in vitro were used to study the CTSB-RNAi-LV mouse brain microvascular endothelial cells (bEND.3), and explore affection of Cathepsin B and capillary-like formation. Results BR>Cell immunohistochemistry showed that Cathepsin B positive profiles appeared brow and were mainly located around the nucleus of bEND.3 cells. Of three groups, expression of Cathepsin B was the least in RNAi lentiviral group. Evaluation of bEND.3 the total protein and capillary-like formation showed that RNAi lentiviral group had less total protein content and formation of capillary-like zone than other groups and there was statistically significant (EM>P /EM><0.05).Conclusion CTSB –RNAi-LV can inhibit expression of Cathepsin B in the bEND.3 cell and formation of capillary-like zone in vitro. CTSB-RNAi-LV can inhibited targeted capillary-like formation EM>