| 油樟叶挥发油及其主要成分的体外抗肝癌活性 |
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| 引用本文： | 叶奎川,殷中琼,魏琴,周黎军,贾仁勇,徐娇,陶翠. 油樟叶挥发油及其主要成分的体外抗肝癌活性[J]. 解剖学报, 2012, 43(3): 381-386. DOI: 10.3969/j.issn.0529-1356.2012.03.017 |
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| 作者姓名： | 叶奎川 殷中琼 魏琴 周黎军 贾仁勇 徐娇 陶翠 |
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| 作者单位： | 1.四川农业大学动物医学院，四川 雅安 625014; 2.宜宾学院西南特色经济植物保护与利用重点实验室，四川 宜宾 644000; 3.四川农业大学动物疫病与人类健康四川省重点实验室，四川 雅安625014 |
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| 基金项目： | 教育部"长江学者和创新团队发展计划"创新团队项目，四川省科技厅支撑项目，宜宾市科技局项目 |
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| 摘 要： | 目的 探讨油樟叶挥发油及其主要组成成分（1,8-桉叶油素、黄樟油素、γ-松油烯、α-松油烯和松油烯-4-醇）的抗肝癌活性。方法 运用体外培养细胞四甲基偶氮唑盐 (MTT)快速比色法，观察油樟叶挥发油的主要组成成分（1,8-桉叶油素、黄樟油素、α-松油烯、松油烯-4-醇和γ-松油烯）对人肝癌BEL-7402细胞的抑制作用，并用软琼脂细胞集落形成实验，观察油樟叶挥发油的主要组成成分对人肝癌BEL-7402细胞形成细胞集落的抑制作用。 结果油樟叶挥发油、1,8-桉叶油素、黄樟油素、γ-松油烯、α-松油烯和松油烯-4-醇体外对人肝癌BEL-7402细胞的最大增殖抑制率分别为58.63%、42.83%、90.04%、81.53%、34.83%和31.46%；对人肝癌BEL-7402细胞集落形成的最大抑制率分别为55.13%、61.99%、94.56%、89.54%、62.02%和59.91%。结论 油樟叶挥发油及其主要组成成分能不同程度地抑制人肝癌BEL-7402细胞的体外增殖及单个细胞形成细胞集落。其中黄樟油素和γ-松油烯对人肝癌BEL-7402细胞的抑制作用最强。
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| 关 键 词： | 油樟叶挥发油 BEL-7402细胞 四甲基偶氮唑盐法 人 |
| 收稿时间： | 2011-10-31 |
| 修稿时间： | 2011-12-08 |
Anticancer activity of the essential oil from Cinnamomum longepaniculatum leaves and its major components against human BEL-7402 |
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| YE Kui-chuan , YIN Zhong-qiong , WEI Qin , ZHOU Li-jun , JIA Ren-yong , XU Jiao , TAO Cui. Anticancer activity of the essential oil from Cinnamomum longepaniculatum leaves and its major components against human BEL-7402[J]. Acta Anatomica Sinica, 2012, 43(3): 381-386. DOI: 10.3969/j.issn.0529-1356.2012.03.017 |
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| Authors: | YE Kui-chuan YIN Zhong-qiong WEI Qin ZHOU Li-jun JIA Ren-yong XU Jiao TAO Cui |
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| Affiliation: | 1. College of Veterinary Medicine Sichuan Agricultural University, Sichuan Ya’an 625014,China; 2.Key Laboratory of Southwest Special Economic Plant Protection and Utilization, Yibin College, Sichuan, Yibin 644000, China; 3.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Sichuan Ya’an 625014, China |
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| Abstract: | Objective In order to study the anticancer activity of essential oil from Cinnamomum longepaniculatum leaves and its major components(1,8-cineole, sassafras oil, γ-terpinene, α-terpinene and terpinene-4-alcohol) against human BEL-7402 ceells. Methods By 3-4,5-diemethylthiarol-2,5-diphenytetrazolium bromide (MTT) fast staining method to study the growth inhibition rate of the essential oil from Cinnamomum longepaniculatum leaves and its major components on human BEL-7402 cells, and by soft agar cloning formation assay to study the effect of the essential oil from Cinnamomum longepaniculatum leaves and its major components against human BEL-7402 cells. Results The growth inhibition rates of the essential oil from Cinnamomum longepaniculatum leaves, 1,8-cineole, sassafras oil, γ-terpinene, α-terpinene and terpinene-4-alcohol against the BEL-7402 cells were 58.63%, 42.83%, 90.04%, 81.53%, 34.83% and 31.46%, respectively in vitro. Colony forming rate of the essential oil from Cinnamomum longepaniculatum leaves, 1,8-cineole, sassafras oil, γ-terpinene. α-terpinene and terpinen-4-alcohol on BEL-7402 cells were 55.13%, 61.99%, 94.56%, 89.54%, 62.02% and 59.91%, respectively. Conclusion The essential oil from Cinnamomum longepaniculatum leaves and its major components can inhibit the proliferation of live |
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| Keywords: | Essential oil of Cinnamomum longepaniculatum BEL-7402 cell MTT Human |
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