参附强心合剂对心衰大鼠水通道蛋白2水平的影响

目的：观察参附强心合剂对心衰大鼠心功能及水通道蛋白2（AQP-2）水平的影响，探讨参附强心合剂抗心衰水潴留的作用机制。方法用腹主动脉缩窄法制作心衰大鼠模型，造模成功60只大鼠随机分为参附强心合剂大、中、小剂量组，氯沙坦组和模型组，每组12只。前三组分别按成人用药剂量20、10、5倍的参附强心合剂14g/（kg·d）、7g/（kg·d）、3.5g/（kg·d）灌胃；氯沙坦组以成人剂量10倍的氯沙坦10mg/（kg·d）灌胃；模型组以蒸馏水灌胃。另假手术组大鼠12只以蒸馏水灌胃作为空白对照组。给药4周后，心超检查测定左室射血分数（LVEF），ELISA法检测大鼠尿AQP-2浓度，Western blot检测大鼠肾脏AQP-2表达水平。结果参附强心合剂可改善心衰大鼠心功能，其各剂量组大鼠尿AQP-2浓度及肾脏AQP-2表达水平均明显下降（P〈0.01或P〈0.05）；随参附强心合剂剂量的增加，AQP-2浓度逐渐下降，EF值逐渐升高；EF值与AQP-2间有良好的线性负性相关关系（P〈0.01）。结论参附强心合剂可有效改善心衰大鼠心功能，降低AQP-2浓度，且呈一定的剂量依赖性；其作用机制与降低心衰大鼠AQP-2浓度有关。

Research on Effects of Shenfu Qiangxin Decoction on Concentration of Urinary Aquaporin 2 in Chronic Heart Failure Rats

Objective To investigate the effect of Shenfu qiangxin（SFQX） decoction on cardiac function and the level of urinary aquaporin 2 in chronic heart failure （CHF） rat model, in an attempt to explore the underlying mechanism of SFQX decoction on water retention in CHF. Methods Sixty CHF models in male adult SD rats were made by coarctation of abdominal aorta （CAA） and then were randomly divided into 5 groups： SFOX groups （large, medium, and low dose）, losarton group, and model group. Another 12 SD rats received sham operation. Large-, medium-, and low-dose SFQX groups were intragastically given 14, 7 and 3.5 g·kg^-1·d^-1 SFQX decoction, losarton group was given 10 mg·kg^-1·d^-1 of losarton, and model group and sham group were grive 2 mL/d of sterile purified water. At the end of 4 weeks, the cardiac function was measured by ultrasonic echocardiogram, and AQP-2 was detected by ELISA and western blot. Results Comparing with model group, the cardiac function were im-proved in SFQX groups;the concentration of urinary AQP-2 and the expression of AQP-2 in renal tissue were re-duced（P〈0.01 or P〈0.05）. The concentration of AQP-2 reduced but ejection fraction（EF） increased correspondingly with the increase of the dose of SFQX decoction. There was a remarkable negative correlation between EF and the concentration of urinary AQP-2 （P〈0.01）. Conclusion SFQX decoction can improve the cardiac function and re-duce the concentration of AQP-2 in CHF rat model in a dose-dependent manner. The mechanism of SFQX decoc-tion on water retention in CHF rats may be related to reduce the concentration of AQP-2.