康莱特联合顺铂高效杀伤骨肉瘤细胞

目的 研究康莱特联合顺铂对骨肉瘤细胞的杀伤作用及其相关机制，探寻骨肉瘤的中西医结合化疗方案。方法 免疫组化及RT—PCR方法检测不同浓度康莱特作用下骨肉瘤OS732细胞的Fas表达，并将康莱特与顺铂单独及联合应用于OS732细胞，采用MTT法检测细胞毒性作用，流式细胞仪定量分析凋亡细胞所占比例，相差显微镜及荧光显微镜下观察凋亡细胞形态改变。结果 康莱特可上调OS732细胞的Fas表达并诱导凋亡，10μL／mL康莱特与1μg／mL顺铂合用于OS732细胞的抑制率与各自单用时相比明显提升（P〈0．01）。细胞形态观察及凋亡率测定也显示，康莱特与顺铂联用可诱导更多OS732细胞凋亡。结论 康莱特可协同顺铂高效杀伤骨肉瘤细胞，这可能与康莱特上调Fas表达诱导骨肉瘤细胞凋亡有关。

Efficient killing effect of osteosarcoma cells induced by combination of Kang-Lai-Te and cisplatin

Objective] To study the killing effect on osteosarcoma cells induced by combination of Kang-Lai-Te and cisplatin and the related mechanism so as to explore the chemotherapeutic method of osteosarcoma with integrated traditional Chinese and western medicine. Methods] Immunohistochemistry and RT-PCR were used to examine the Fas expression of OS732 cells, Kang-Lai-Te and cisplatin were applied on OS732 cells respectively or jointly and the inhibition rates were measured by MTT assay. The changes of cellular shape were observed with inverted phase contrast microscope and fluorescence microscope. The apoptotic rates were analyzed with flow cytometry (FCM). Results] Kang-Lai-Te has the effect of up-regulating Fas expression and inducing apoptosis. The inhibition rate of combined application of 10 μl/mL Kang-Lai-Te and 1 μg/mL cisplatin on OS-732 cells was significantly higher than that of the respective application(P ＜0.01). Changes of cellular shape and apoptotie rates also indicated the apoptosis-inducing effect of combined application was much stronger than that of respective application.Conclusion] Combination of Kang-Lai-Te and cisplatin shows a strong killing effect on OS-732 cells which might be related to the up-regulation of Fas expression by Kang-Lai-Te.