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| 少精症患者Y染色体微缺失基因诊断的研究 | |
| 引用本文： | 钱卫平,李崎,谭玉梅,邹红艳,张敏,宋丹,卢光琇.少精症患者Y染色体微缺失基因诊断的研究[J].实用预防医学,2005,12(3):566-568. |
| 作者姓名： | 钱卫平 李崎 谭玉梅 邹红艳 张敏 宋丹 卢光琇 |
| 作者单位： | 1. 中南大学生殖与干细胞工程研究所,湖南,长沙,410008;深圳市罗湖医院生殖医学中心 2. 深圳市罗湖医院生殖医学中心 3. 中南大学生殖与干细胞工程研究所,湖南,长沙,410008 |
| 摘 要： | 目的 建立稳定的少、弱精症患者Y染色体微缺失的基因诊断方法，研究 男性不育与Y染色体位点缺失的相关性。方法选取位于AZE区15个STR微卫星标记分成4组进行多重PCR的检测。结果 90例少弱精患者中，检出7例缺失，占患者的7.8％。其中有3例为单个位点的缺失，有4例为大片断缺失。结论 多重PCR是检测Y染色体微缺失的合适方法，AZEb和AZEc区与少弱精症密切相关。 |
| 关 键 词： | 男性不育 Y染色体 多重PCR 微缺失 |
| 文章编号： | 1006-3110（2005）03-0566-03 |
| 收稿时间： | 2005-03-08 |
| 修稿时间： | 2005年3月8日 |
Genetic Diagnosis of the Microdeletions in the Y Chromosome of Idiopathic Oiigospermia or Azoospermia |
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| QIAN Wei - ping, LI Qi , TAN Yu mei ,et al..Genetic Diagnosis of the Microdeletions in the Y Chromosome of Idiopathic Oiigospermia or Azoospermia[J].Practical Preventive Medicine,2005,12(3):566-568. | |
| Authors: | QIAN Wei - ping LI Qi TAN Yu mei |
| Institution: | Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha 410008, Hunan |
| Abstract: | Objective To create genetic diagnosis method of the microdeletions in the Y chromosome, to assess the relationships between idiopathic oligospermia or azoospermia and microdeletions in the Y chromosome. Methods Fifteen Y linked sequence tagged sites in AZF region which divided into 4 groups and were screened by means of multiplex PGR in 90 idiopathic oligospermia or azoospermia patients. Results Microdeletions in the genomic DNA were observed in 7 of the 90 cases , the total deletion rate was 7.8 %. Among them, .3 cases had single site deletion,4 cases had multiplex sites deletions. Conclusions Multiplex PCR is a valid technique for detection of microdeletions. Microdeletions of the AZFb and AZFc may be an important reason of idiopathic oligospermia or azoospermia. |
| Keywords: | Infertility male Y chromosome Multiplex PCR Microdeletion |
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