胆囊腺癌中KAI 1mRNA、Kiss 1mRNA表达的临床病理意义

目的 研究胆囊腺癌、慢性胆囊炎和胆囊结石组织中KAI 1mRNA和Kiss 1mRNA表达水平及其临床病理意义。方法 108例胆囊腺癌、15例慢性胆囊炎和20例胆囊结石组织标本常规制作石蜡包埋切片，KAI 1mRNA和Kiss 1mRNA染色方法为原位杂交染色法。结果 胆囊腺癌组织中KAI 1mRNA和Kiss 1mRNA阳性表达率明显低于慢性胆囊炎和胆囊结石组织（P＜0.05，P＜0.01）；腺瘤癌变或高分化腺癌、肿块最大径≤3cm、无淋巴结转移及未侵犯周围组织的病例KAI 1mRNA和Kiss 1mRNA阳性表达率明显高于低分化腺癌或粘液腺癌、肿块最大径＞3cm、淋巴结转移及侵犯周围组织的病例（P＜0.05，P＜0.01）。结论 KAI 1mRNA和Kiss 1mRNA表达可能是反映胆囊腺癌发生、进展、转移或侵袭潜力及预后的重要生物学标记物。

Expression of KAI-1mRNA, Kiss-1mRNA and Its Clinicopathological Significance in Gall-bladder Adenocarcinoma

Objective 　To study on the expression of KAI21 mRNA and Kiss21 mRNA and detect their clin2 icopathological significances in gallbladder adenocarcinoma tissues. Methods 　In situ hybridization was used for detecting the expression levels of KAI21 mRNA and Kiss21 mRNA on routinely paraffin2embed2 ded sections f rom 108 cases with gallbladder adenocarcinoma , 15 ones with chronic cholecystitis and 20 ones with gallstone. Results 　The positive rates of KAI21 mRNA and Kiss21 mRNA were significantly lower in gallbladder adenocarcinoma than those in chronic cholecystitis and gallstone tissues. The positive rates of KAI21mRNA and Kiss21mRNA were significantly higher in the cases of adenomatous canceration or high2differentiated adenocarcinoma , maximum diameter of mass ≤3 cm , without2metastasis of lymph node , or without2infilt ration of regional tissues than those in ones of low2differentiated carcinoma or mu2 cinous adenocarcinoma , maximum diameter of mass > 3 cm , with2metastasis of lymph node or with2infil2 t ration of regional tissues in gallbladder adenocarcinoma tissues. Conclusion 　The expression of KAI21 mRNA and Kiss21 mRNA might be important biological markers for reflecting the carcinogenesis , pro2 gression , metastasis or infilt rating potential and prognosis of gallbladder adenocarcinoma.