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| 灭活SARS冠状病毒疫苗对小鼠脏器影响的实验研究 | |
| 引用本文： | 杜彬,钟雪云,熊盛,张传海,刘新建,刘石生,张美英,李久香,王一飞,陆家海,万卓越,鄢心革,郑焕英,范江林. 灭活SARS冠状病毒疫苗对小鼠脏器影响的实验研究[J]. 中国病理生理杂志, 2004, 20(7): 1187-1189 |
| 作者姓名： | 杜彬 钟雪云 熊盛 张传海 刘新建 刘石生 张美英 李久香 王一飞 陆家海 万卓越 鄢心革 郑焕英 范江林 |
| 作者单位： | 1. 暨南大学医学院病理解剖教研室, 广东 广州 510632; 2. 暨南大学生物医药研究开发基地, 广东 广州 510632; 3. 中山大学公共卫生学院, 广东 广州 510089; 4. 广东省疾病预防控制中心, 广东 广州 510300; 5. 日本筑波大学 |
| 基金项目： | 广东省非典科技攻关专项 (No .2 0 0 32 3-E -4 6 1)，教育部科技研究重点项目 (No .10 4 181)，广东省自然科学基金重点项目 (No . 0 132 0 3)，广州市科技攻关项目(No .2 0 0 3Z3-E0 4 0 1) |
| 摘 要： | 目的:探讨灭活SARS冠状病毒疫苗对小鼠是否有损伤作用。方法:用灭活SARS冠状病毒分别免疫BALB/c和C57BL/6小鼠;ELISA法检测小鼠血清中抗SARS冠状病毒抗体水平;免疫8周后取小鼠各脏器,观察其病理形态学改变。结果:免疫8 d后,小鼠血清中就可检测到特异的IgM、IgG抗体;组织切片染色观察,可见少数小鼠肺及肝组织出现轻度损伤,其他脏器未见病变。结论:SARS冠状病毒灭活疫苗可诱导小鼠产生特异性抗体,同时没有造成严重的器官损伤。这将为SARS疫苗进入临床研究打下基础。 |
| 关 键 词： | 严重急性呼吸综合征 冠状病毒 疫苗 灭活 小鼠 |
| 文章编号： | 1000-4718(2004)07-1187-03 |
| 收稿时间： | 2004-02-27 |
| 修稿时间： | 2004-04-16 |
Effect of inactivated SARS coronavirus vaccine on mouse organs |
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| DU Bin,ZHONG Xue-yun,XIONG Sheng,ZHANG Chuan-hai,LIU Xin-jian,LIU Shi-sheng,ZHANG Mei-ying,LI Jiu-xiang,WANG Yi-fei,LU Jia-hai,WAN Zhuo-yue,YAN Xin-ge,ZHENG Huan-ying,FAN Jiang-lin. Effect of inactivated SARS coronavirus vaccine on mouse organs[J]. Chinese Journal of Pathophysiology, 2004, 20(7): 1187-1189 | |
| Authors: | DU Bin ZHONG Xue-yun XIONG Sheng ZHANG Chuan-hai LIU Xin-jian LIU Shi-sheng ZHANG Mei-ying LI Jiu-xiang WANG Yi-fei LU Jia-hai WAN Zhuo-yue YAN Xin-ge ZHENG Huan-ying FAN Jiang-lin |
| Affiliation: | 1. Department of Pathology, Medical College, Jinan University, Guangzhou 510632, China;; 2. Biomedical Research & Development Center, Jinan University, Guangzhou 510632, China; 3. School of Public Health, Sun Yat sen University, Guangzhou 510089, China; 4. Center of Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China; 5. University of Tsukuba, Japan |
| Abstract: | AIM: To study the pathological change in mouse organs immunitied by inactivated SARS-CoV vaccine. METHODS: Inactivated SARS-CoV vaccine was injected into BALB/c and C57BL/6 mice. Anti-SARS antibody was analyzed by ELISA. After 8 weeks, the immunitied mice were killed and those organs were analyzed by pathological methods. RESULTS: Anti-SARS antibody in mice was positive after 8 days. Only minimal injury was observed in a few lungs and livers, but the other organs were not. CONCLUSIONS: Inactivated SARS-CoV vaccine induced mice to create antibody, whereas they did not cause severe injury. This result will be valuable for vaccine into clinical research. [ |
| Keywords: | Severe acute respiratory syndrome Coronavirus Vaccines inactivated Mice |
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