谷氨酰胺增强氧化应激时肺微血管内皮细胞热休克蛋白70的表达

目的研究谷氨酰胺（Gln）对氧化应激状态下体外培养肺微血管内皮细胞（pulmonary microvascular endothelial cells，PMVEC）热休克蛋白（HSP）70表达的影响。方法采用体外培养Wistar大鼠PMVEC，分为阳性对照组（G组）、生理盐水组（PS组）、谷氨酰胺组（Gln组）、过氧化氢加生理盐水组（H₂O₂+PS组）和过氧化氢加谷氨酰胺组（H₂O₂+Gln组）。Western blotting检测PMVEC HSP70的表达情况，分别记录平均灰度值。结果与PS组（9.05±1.51）相比，Gln组（40.28±10.19）和H₂O₂+PS组（43.89±10.32）PMVEC HSP70表达分别增高4.5倍和5倍（P＜0.01）；与H₂O₂+PS组相比，H₂O₂+Gln组（65.59±14.53）HSP70表达进一步增高，约为PS组的7倍，接近G组（70.56±12.23）表达水平。结论谷氨酰胺可增强氧化应激状态下体外培养肺微血管内皮细胞HSP70表达。

Glutamine enhances the expression of heat shock protein 70 in pulmonary microvascular endothelial cells under oxidative stress conditions

ObjectiveTo investigate the effects of glutamine on the expression of heat shock protein (HSP) 70 in pulmonary micro-vascular endothelial cells (PMVECs) under oxidative stress conditions. MethodsPMVECs from Wistar rats were divided into 5 groups: the positive control group (G group), the physiologic saline group (PS group), the glutamine group(Gln group), the H₂O₂+PS group and the H₂O₂+Gln group. Western blotting was used to investigate the expression of HSP70. ResultsCompared with the PS group, the expression of HSP70 significantly increased in the Gln group（9.05±1.51 vs 40.28±10.19） and the H2O2+PS group（9.05±1.51 vs 43.89±10.32）. The expression of HSP70 was higher in the H₂O₂+Gln group than in the H₂O₂+PS group（65.59±14.53 vs 43.89±10.32）. ConclusionGlutamine enhances the expression of HSP70 in pulmonary micro-vascular endothelial cells under oxidative stress conditions.