| 肾上腺髓质素真核表达载体对皮瓣缺血再灌注损伤的影响 |
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| 引用本文： | 杨锋,刘志坤,何葵.肾上腺髓质素真核表达载体对皮瓣缺血再灌注损伤的影响[J].中国组织工程研究与临床康复,2011,15(28). |
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| 作者姓名： | 杨锋 刘志坤 何葵 |
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| 作者单位： | 1. 南华大学附属第二医院整形外科,湖南省衡阳市,421001
2. 衡阳市中心医院,湖南省衡阳市,421001 |
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| 基金项目： | 课题受衡阳市科技局项目科研基金资助项目(2009KJ47)资助;项目名称:重组肾上腺髓质素对大鼠皮瓣缺血再灌注损伤的保护作用 |
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| 摘 要： | 背景:肾上腺髓质素可在心、脑、肾、肝等器官的缺血再灌注过程中起保护作用.目的:观察肾上腺髓质素真核表达载体对大鼠腹部皮瓣缺血再灌注损伤的影响及其作用机制.方法:构建大鼠肾上腺髓质素真核表达载体,将SD大鼠随机分假手术组、模型组、维拉帕米组、重组质粒组.重组质粒组腹部皮内注射肾上腺髓质素真核表达载体,其余各组注射等量生理盐水,各组注射4次后建立缺血再灌注模型,维拉帕米组于皮瓣内注射维拉帕米.结果与结论:与模型组相比,重组质粒组皮瓣组织中丙二醛、血管内皮素1的含量分别降低了38.50%(P < 0.01),54.73% (P < 0.01),超氧化物歧化酶的含量增加了50.67%(P < 0.05).皮瓣组织学检查结果显示重组质粒组皮瓣炎性细胞浸润与水肿程度也较模型组明显减轻.结果证实肾上腺髓质素真核表达载体对大鼠皮瓣缺血再灌注损伤具有保护作用,其机制与减轻脂质过氧化及减少炎症细胞浸润有关.
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| 关 键 词： | 肾上腺髓质素 缺血再灌注 真核表达 皮瓣 组织构建 |
Effects of adrenomedullin eukaryotic expression vector on skin flap ischemia reperfusion injury |
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| Yang Feng,Liu Zhi-kun,He Kui.Effects of adrenomedullin eukaryotic expression vector on skin flap ischemia reperfusion injury[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(28). |
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| Authors: | Yang Feng Liu Zhi-kun He Kui |
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| Institution: | Yang Feng1,Liu Zhi-kun1,He Kui2 1Department of Plastic Surgery,the Second Affiliated Hospital of University of South China,Hengyang 421001,Hunan Province,China,2Hengyang Central Hospital |
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| Abstract: | BACKGROUND:Adrenomedullin plays protective effects on ischemia and reperfusion injury of the heart, brain, kidney and liver. OBJECTIVE:To explore the effects of adrenomedullin eukaryotic expression vector pVAX1-ADM on ischemia reperfusion injury in rat abdominal flaps and the possible mechanism of action. METHODS:Rat adrenomedullin eukaryotic expression vector pVAX1-ADM was reconstructed. Rats were randomly divided into four groups:sham-operated, model, Verapamil, and pVAX1-ADM. Rats in the pVAX1-ADM group ... |
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