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| 密度梯度离心法体外培养骨髓间充质干细胞的分化能力 | |
| 引用本文： | 穆晓红,赵子义,徐林,王硕仁,朱陵群,刘铜华. 密度梯度离心法体外培养骨髓间充质干细胞的分化能力[J]. 中国组织工程研究与临床康复, 2011, 15(27). DOI: 10.3969/j.issn.1673-8225.2011.27.004 |
| 作者姓名： | 穆晓红 赵子义 徐林 王硕仁 朱陵群 刘铜华 |
| 作者单位： | 1. 北京中医药大学,北京市,100029 2. 北京中医药大学东直门医院骨科,北京市,100700 3. 北京中医药大学中心实验室,北京市,100700 |
| 基金项目： | 国家自然科学基资助项目 |
| 摘 要： | 背景:骨髓间充质干细胞具有多向分化能力,是目前极具潜力的种子细胞及基因治疗的靶细胞.目的:体外培养兔骨髓来源间充质干细胞,了解其生物学特性.方法:采用密度梯度离心法培养兔骨髓间充质干细胞,对细胞的形态及生长特性进行观察,应用流式细胞仪检测细胞表面抗原CD29、CD34、CD44、CD45、CD166、HLA-DR 表达,并进行相关生物学特性鉴定.结果与结论:密度梯度离心法能分离培养出纯度较高的骨髓间充质干细胞,流式细胞仪检测骨髓间充质干细胞表达CD29、CD44、CD166,不表达CD34、CD45、HLA-DR.在适当的条件下能够诱导分化成为成骨细胞、软骨细胞等. |
| 关 键 词： | 骨髓间充质干细胞 分离 培养 分化 生物学特性 |
Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro |
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| Mu Xiao-hong,Zhao Zi-yi,Xu Lin,Wang Shuo-ren,Zhu Ling-qun,Liu Tong-hua. Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(27). DOI: 10.3969/j.issn.1673-8225.2011.27.004 | |
| Authors: | Mu Xiao-hong Zhao Zi-yi Xu Lin Wang Shuo-ren Zhu Ling-qun Liu Tong-hua |
| Affiliation: | Mu Xiao-hong1,Zhao Zi-yi2,Xu Lin2,Wang Shuo-ren3,Zhu Ling-qun3,Liu Tong-hua1 1Beijing University of Chinese Medicine,Beijing 100029,China,2Department of Orthopaedics,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,3Central Laboratory |
| Abstract: | BACKGROUND:Bone marrow mesenchymal stem cells(BMSCs) are capable of multiple directional differentiation,and are potential seed cells and target cells for gene therapy.OBJECTIVE:To observe in vitro cultured rabbit BMSCs,to understand its biological properties.METHODS:Density gradient centrifugation cultivation was utilized to culture rabbit BMSCs.The morphology and characteristics of cultured cells were observed.The surface markers CD29,CD34,CD44,CD45,CD166 and HLA-DR of cells were identified by flow cytome... |
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