| 引产儿与正常成人骨髓间充质干细胞的比较 |
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| 引用本文： | 程月爱,刘瑞风,张永翠,张开明. 引产儿与正常成人骨髓间充质干细胞的比较[J]. 中国组织工程研究与临床康复, 2011, 15(32). DOI: 10.3969/j.issn.1673-8225.2011.32.009 |
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| 作者姓名： | 程月爱 刘瑞风 张永翠 张开明 |
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| 作者单位： | 太原市中心医院皮肤科,山西省太原市,030009 |
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| 基金项目： | 国家自然科学基金项目项目，课题名称:银屑病患者骨髓造血干细胞定向分化为 T 细胞过程Notch 信号转导通路的动态研究 |
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| 摘 要： | 背景:骨髓间充质干细胞是多能干细胞,同时可参与免疫调节反应,而成人及引产胎儿骨髓均是间充质干细胞的重要来源.目的:通过引产儿与正常成人骨髓间充质干细胞的细胞形态、免疫表型、增殖活性及分泌细胞因子的比较,揭示二者生物学特点的差异.方法:采用密度梯度离心法分离引产儿与正常成人骨髓单个核细胞,通过贴壁法培养骨髓间充质干细胞,收集传3代后的骨髓间充质干细胞,流式细胞术鉴定其免疫表型、MTT比色法检测其增殖活性以及用ELISA法检测细胞培养上清液中白细胞介素6、血小板源性生长因子和表皮生长因子的水平.结果与结论:引产儿组和正常成人组骨髓间充质干细胞形态相似、表型相同,分泌细胞因子水平无显著差异,但引产儿细胞增殖活性显著高于正常成人.提示引产儿与正常成人骨髓间充质干细胞除增殖活性有差异外,其余生物学特点相似,引产儿可以为间充质干细胞研究提供新的骨髓来源.
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| 关 键 词： | 引产儿 成人 骨髓 间充质干细胞 生物学特点 |
Comparison of bone marrow-derived mesenchymal stem cells from aborted fetus and normal adult subjects |
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| Cheng Yue-ai,Liu Rui-feng,Zhang Yong-cui,Zhang Kai-ming. Comparison of bone marrow-derived mesenchymal stem cells from aborted fetus and normal adult subjects[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(32). DOI: 10.3969/j.issn.1673-8225.2011.32.009 |
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| Authors: | Cheng Yue-ai Liu Rui-feng Zhang Yong-cui Zhang Kai-ming |
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| Affiliation: | Cheng Yue-ai,Liu Rui-feng,Zhang Yong-cui,Zhang Kai-ming Department of Dermatology,Taiyuan Central Hospital,Taiyuan 030009,Shanxi Province,China |
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| Abstract: | BACKGROUND: Bone marrow-derived mesenchymal stem cells are pluripotent cells and can be involved in immune response. Fetal and adult bone marrow is both important sources for mesenchymal stem cells. OBJECTIVE: To reveal the differences of bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects by comparing the biological characters (including morphology, immunophenotype, proliferative activity and cytokines secretion). METHODS: Bone marrow mononuclear cells of aborted fetus a... |
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