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| 胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节 | |
| 引用本文： | 郭振兴,郑翠玲,陈振萍,董文川,杨仁池. 胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节[J]. 中国组织工程研究与临床康复, 2011, 15(32): 5901-5904. DOI: 10.3969/j.issn.1673-8225.2011.32.003 |
| 作者姓名： | 郭振兴 郑翠玲 陈振萍 董文川 杨仁池 |
| 作者单位： | 1. 清华大学第一附属医院血液肿瘤科,北京市,100016 2. 中国医学科学院肿瘤医院检验科,北京市,100029 3. 北京儿童医院血液中心,北京市,100045 4. 中国医学科学院血液学研究所实验血液学国家重点实验室,天津市,300020 |
| 摘 要： | 背景:众多研究表明间充质干细胞能发挥免疫调节功能,抑制T细胞增殖。目的:观察胚胎骨髓来源间充质干细胞对人Th17细胞的调节作用。方法:将人胚胎骨髓间充质干细胞与正常人外周血单个核细胞或CD4+T细胞以1∶10比例共培养4d,以单个核细胞或CD4+T细胞单独培养为对照。应用实时定量PCR检测细胞白细胞介素17mRNA表达,酶联免疫吸附试验检测细胞上清中白细胞介素17蛋白水平,流式细胞术检测Th17细胞数量。结果与结论:胚胎骨髓来源间充质干细胞与单个核细胞共培养组白细胞介素17mRNA表达水平明显高于单个核细胞组(P<0.01)。与此一致的是,胚胎骨髓来源间充质干细胞与单个核细胞或CD4+T细胞共培养组细胞上清中白细胞介素17蛋白水平明显高于单个核细胞组、CD4+T细胞组(P<0.05,P<0.01)。胚胎骨髓来源间充质干细胞与CD4+T细胞共培养组Th17细胞数量明显高于CD4+T细胞组(P<0.01),但胚胎骨髓来源间充质干细胞本身并不表达白细胞介素17。表明胚胎骨髓来源间充质干细胞可促进人Th17细胞增殖。 |
| 关 键 词： | 胚胎 骨髓 间充质干细胞 Th17细胞 免疫调节 |
Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells |
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| Guo Zhen-xing,Zheng Cui-ling,Chen Zhen-ping,Dong Wen-chuan,Yang Ren-chi. Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(32): 5901-5904. DOI: 10.3969/j.issn.1673-8225.2011.32.003 | |
| Authors: | Guo Zhen-xing Zheng Cui-ling Chen Zhen-ping Dong Wen-chuan Yang Ren-chi |
| Affiliation: | Guo Zhen-xing1,Zheng Cui-ling2,Chen Zhen-ping3,Dong Wen-chuan1,Yang Ren-chi4 1Department of Hematology/Oncology,First Hospital of Tsinghua University,Beijing 100016,China,2Department of Clinical Laboratory,Cancer Hospital,Chinese Academy of Medical Sciences,Beijing 100021,3Hematology Center,Beijing Children's Hospital,Beijing 100045,4State Key Laboratory of Experimental Hematology,Institute of Hematology & Blood Diseases Hospital,Tianjin 30... |
| Abstract: | BACKGROUND: Many studies have demonstrated mesenchymal stem cell (MSC) can regulate the immune function and inhibit proliferation of T cell. OBJECTIVE: To investigate the regulatory function of fetal bone marrow-derived mesenchymal stem cells (FBM-MSCs) on human Th17 cells. METHODS: FBM-MSCs were cocultured with human peripheral blood mononuclear cells (PBMCs) or CD4+ T cells at 1:10 ratio from healthy donors for 4 days. Single culture of mononuclear cells or CD4+ T cells were as controls. The expression of... |
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