| 全骨髓贴壁法分离培养大鼠骨髓间充质干细胞及其生物学特性 |
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| 引用本文： | 赵林,冯智慧,焦淑贤,李南南.全骨髓贴壁法分离培养大鼠骨髓间充质干细胞及其生物学特性[J].中国组织工程研究与临床康复,2011,15(32). |
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| 作者姓名： | 赵林 冯智慧 焦淑贤 李南南 |
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| 作者单位： | 1. 青岛市中心血站输血医学研究所,山东省青岛市,266071
2. 大连医科大学,辽宁省大连市,116027 |
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| 基金项目： | 青岛市科技发展计划，课题名称:骨髓基质干细胞对失代偿性病毒性肝炎修复的中长期影响 |
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| 摘 要： | 背景:培养出生长状态良好、数量足够多的大鼠骨髓间充质干细胞是其作为种子细胞在组织工程等领域广泛应用的重要前提.目的:寻求快捷有效的大鼠骨髓间充质干细胞体外培养条件,观察培养的间充质干细胞生物学特性.方法:全骨髓法体外分离培养大鼠骨髓间充质干细胞,贴壁筛选法进行纯化,倒置相差显微镜下观察细胞形态及生长特征,免疫荧光分析细胞骨架,MTT法绘制细胞生长曲线,流式细胞仪检测其表面标记.结果与结论:分离培养的细胞呈长梭形或多边形;细胞生长曲线呈S形;微丝免疫荧光染色结果显示,培养的骨髓间充质干细胞具有良好的细胞骨架系统.第3代骨髓间充质干细胞CD29、CD44、CD71、CD90均呈阳性表达,而CD13、CD34、CD45、CD133呈阴性.提示,经全骨髓贴壁法体外分离培养的细胞在形态学和细胞表面标志物表达方面具有干细胞生物学特性,经鉴定为骨髓间充质干细胞,其第3代活性最佳,可用于后续实验.
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| 关 键 词： | 骨髓间充质干细胞 大鼠 贴壁筛选法 分离培养 生物学特性 |
Culture condition and biological characteristics of rat bone marrow mesenchymal stem cells by using the whole bone marrow adherence method |
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| Zhao Lin,Feng Zhi-hui,Jiao Shu-xian,Li Nan-nan.Culture condition and biological characteristics of rat bone marrow mesenchymal stem cells by using the whole bone marrow adherence method[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(32). |
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| Authors: | Zhao Lin Feng Zhi-hui Jiao Shu-xian Li Nan-nan |
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| Institution: | Zhao Lin1,Feng Zhi-hui1,Jiao Shu-xian1,Li Nan-nan2 1Institute of Transfusion Medicine,Qingdao Blood Center,Qingdao 266071,Shandong Province,China,2Dalian Medical University,Dalian 116027,Liaoning Province |
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| Abstract: | BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are widely used as seed cells in tissue engineering and other fields. Therefore, well growth and enough number of rat BMSCs are important for above-mention studies. OBJECTIVE: To explore the suitable primary culture condition of rat BMSCs in vitro, and to observe the growth property of BMSCs. METHODS: BMSCs were separated using whole bone marrow culture method and purified using attachment method. Morphology and growth characteristics were observed unde... |
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