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| 骨髓间充质干细胞修复损伤脊髓的实验及临床应用短期随访 | |
| 引用本文： | 高德萱,贾全章,李东君,陈玉丙,孙景海,王风华,徐爽,刘丽萍,姜大伟.骨髓间充质干细胞修复损伤脊髓的实验及临床应用短期随访[J].中国组织工程研究与临床康复,2011,15(27):5016-5020. |
| 作者姓名： | 高德萱 贾全章 李东君 陈玉丙 孙景海 王风华 徐爽 刘丽萍 姜大伟 |
| 作者单位： | 1. 延边大学医学部,吉林省延吉市,133002 2. 解放军第208,医院,吉林省长春市130062 3. 吉林大学附属第二医院,吉林省长春市,130000 |
| 基金项目： | 吉林省科技发展计划项目(20080446)"骨髓间充质干细胞对脊髓损伤模型大鼠的修复作用及临床应用研究" |
| 摘 要： | 背景:骨髓间充质干细胞治疗脊髓损伤的研究已经逐渐由动物实验过渡到临床,但其作用机制还不完全清楚。目的:观察骨髓间充质干细胞移植对脊髓损伤大鼠脊髓功能的修复作用,并通过临床应用观察短期疗效。方法:采用改良Allen’s打击法造成Wistar大鼠脊髓损伤模型,将体外分离培养的骨髓间充质干细胞分别经尾静脉及损伤局部移植,应用改良Tarlov评分评定大鼠行为学变化,在光镜下对损伤脊髓病理切片进行对比分析。对5例脊髓损伤患者通过损伤原位注射、腰穿、静脉输注的方式行人自体骨髓间充质干细胞移植,并行神经功能及生活能力评定。结果与结论:治疗后15d,骨髓间充质干细胞尾静脉及损伤局部移植组大鼠运动功能评分较模型对照组显著提高;移植后7,15,30d,脊髓病理切片显示骨髓间充质干细胞尾静脉及损伤局部移植组大鼠较模型对照组有显著恢复。临床患者骨髓间充质干细胞移植后半年神经功能及生活能力均有改善。 |
| 关 键 词： | 脊髓损伤 骨髓间充质干细胞 修复 神经功能 生活能力 |
Experimental study and short-term clinical follow-up of bone mesenchymal stem cells transplantation for repair of spinal cord injury |
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| Gao De-xuan,Jia Quan-zhang,Li Dong-jun,Chen Yu-bing,Sun Jing-hai,Wang Feng-hua,Xu Shuang,Liu Li-ping,Jiang Da-wei.Experimental study and short-term clinical follow-up of bone mesenchymal stem cells transplantation for repair of spinal cord injury[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(27):5016-5020. | |
| Authors: | Gao De-xuan Jia Quan-zhang Li Dong-jun Chen Yu-bing Sun Jing-hai Wang Feng-hua Xu Shuang Liu Li-ping Jiang Da-wei |
| Institution: | Gao De-xuan1,Jia Quan-zhang2,Li Dong-jun1,Chen Yu-bing3,Sun Jing-hai2,Wang Feng-hua2,Xu Shuang2,Liu Li-ping3,Jiang Da-wei1 1Medical School of Yanbian University,Yanji 133002,Jilin Province,China,2The 208 Hospital of Chinese PLA,Changchun 130062,3Second Affiliated Hospital of Jilin University,Changchun 130000 |
| Abstract: | BACKGROUND:Studies of bone marrow mesenchymal stem cells(BMSCs) have gradually experienced a transition from animal experiments to clinical application.However,the mechanism is still unclear.OBJECTIVE:To observe the repairing effect and short-term effect of BMSCs transplantation on rats motor function.METHODS:Spinal cord injury models were prepared by modified Allen's crack method;the BMSCs which were isolated and cultured in vitro were transplanted into the model through the vena caudalis and the local les... |
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