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| 猪脂肪干细胞与脱细胞真皮基质的生物相容性 | |
| 引用本文： | 赵宝成,王振军,韩加刚,顾蓓,徐慧民,喻学桥. 猪脂肪干细胞与脱细胞真皮基质的生物相容性[J]. 中国组织工程研究与临床康复, 2011, 15(27): 4988-4992. DOI: 10.3969/j.issn.1673-8225.2011.27.011 |
| 作者姓名： | 赵宝成 王振军 韩加刚 顾蓓 徐慧民 喻学桥 |
| 作者单位： | 1. 首都医科大学附属北京朝阳医院普外科,北京市,100020 2. 中国医学科学院基础医学研究所,北京市,100730 3. 潍坊市人民医院普外科,山东省潍坊市,261041 |
| 基金项目： | 北京市卫生系统高层次卫生技术人才，国家级新世纪百千万人才工程 |
| 摘 要： | 背景:脱细胞真皮基质已广泛应用于器官或组织缺损的修补。目的:观察猪脂肪源性干细胞与脱细胞真皮基质的相容性。方法:酶消化法原代培养猪脂肪源性干细胞,利用流式细胞仪和多向诱导分化方法鉴定脂肪干细胞,将其与脱细胞真皮基质共培养。结果与结论:实验成功培养出猪脂肪源性干细胞,流式细胞仪检测结果显示阳性表达CD44和CD105,不表达CD34和CD45;在诱导培养基条件下,可向脂肪细胞、成骨细胞分化。苏木精-伊红染色及扫描电镜发现干细胞能在脱细胞真皮基质表面黏附生长。脂肪源性干细胞与脱细胞真皮基质具有良好的相容性,将两者体外复合培养后,有望将复合物植入体内进行缺损组织、器官的修复。 |
| 关 键 词： | 脱细胞真皮基质 脂肪源性干细胞 酶消化法 培养 生物相容性 猪 组织工程 |
Biocompatibility of procine adipose derived stem cells and acellular dermal matrix |
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| Zhao Bao-cheng,Wang Zhen-jun,Han Jia-gang,Gu Bei,Xu Hui-min,Yu Xue-qiao. Biocompatibility of procine adipose derived stem cells and acellular dermal matrix[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(27): 4988-4992. DOI: 10.3969/j.issn.1673-8225.2011.27.011 | |
| Authors: | Zhao Bao-cheng Wang Zhen-jun Han Jia-gang Gu Bei Xu Hui-min Yu Xue-qiao |
| Affiliation: | Zhao Bao-cheng1,Wang Zhen-jun1,Han Jia-gang1,Gu Bei2,Xu Hui-min3,Yu Xue-qiao1 1Department of General Surgery,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China,2Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Beijing 100730,3Department of General Surgery,Weifang People's Hospital,Weifang 261041,Shandong Province |
| Abstract: | BACKGROUND:Acellular dermal matrix has been widely used as a repair material in organ and tissue repair.OBJECTIVE:To investigate the biocompatibility of procine adipose derived stem cells and acellular dermal matrix.METHODS:Porcine adipose derived stem cells were obtained and cultured with collagenase digestion method.Flow cytometric analysis and adipogenic,octeogenic differentiation analysis were used to identify the stem cells.RESULTS AND CONCLUSION:The stem cells isolated from subcutaneous adipose tissue... |
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