| IL—2—绿脓杆菌外毒素融合蛋白增强杀伤细胞活性 |
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| 引用本文： | 高基民,郑仲承.IL—2—绿脓杆菌外毒素融合蛋白增强杀伤细胞活性[J].中华微生物学和免疫学杂志,1996,16(1):37-40. |
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| 作者姓名： | 高基民 郑仲承 |
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| 作者单位： | 第一军医大学免疫学教研室!广州 510515(高基民，林来兴妹，王小宁)，中国科学院上海生物化学研究所(徐舲飞，郑仲承，刘新垣) |
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| 基金项目： | 本课题受“863”、国家自然科学基金，全军青年科学基金，广东省青年科学基金资助 |
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| 摘 要： | 实验证实了白细胞介素２－绿脓杆菌外毒素融合蛋白ＩＬ２－ＰＥ４０ＲＥＤＬＫ、ＩＬ２－ＰＥ４０ＫＤＥＬ、ＩＬ２－ＰＥ６６4GluＲＥＤＬＫ、ＩＬ２－ＰＥ６６4GluＫＤＥＬ对含高亲和力ＩＬ－２受体靶细胞的杀伤效应是借助于ＩＬ－２与其受体的特异性结合和ＰＥ的杀伤活性介导的，而且同一靶细胞对这些融合蛋白的敏感性有较大的差异，对人外周血ＰＨＡ体外活化的淋巴母细胞的杀伤活性，ＩＬ２－ｐＥ６６4GluＫＤＥＬ要比ＩＬ２－ＰＥ４０ＲＥＤＬＫ强约４０倍，还发现若将ＩＬ２－ＰＥ融合蛋白羧基末端的ＲＥＤＬＫ氨基酸突变为ＫＤＥＬ可增强其杀伤细胞的活性，对ＣｏｎＡ活化的小鼠脾母细胞的杀伤活性，ＩＬ２－ＰＥ４０ＫＤＥＬ要比ＩＬ２－ｐＥ４０ＲＥＤＬＫ强８倍。
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| 关 键 词： | 白细胞介素2 绿脓杆菌外毒素 融合蛋白 细胞毒活性 定位诱变 |
Enhancement of cytotoxicitic activity of interleukin 2-Pseudomonas fusion proteins |
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| Gao Jimin.,Xu Ling fei,Zheng Zhongcheng,et al.Enhancement of cytotoxicitic activity of interleukin 2-Pseudomonas fusion proteins[J].Chinese Journal of Microbiology and Immunology,1996,16(1):37-40. |
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| Authors: | Gao Jimin Xu Ling fei Zheng Zhongcheng |
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| Abstract: | We confirmed that the IL 2-PE fusion proteins such as IL2-PE4SREDLK , IL2- PE40KDEL ,IL2-PE66 REDLK and IL2-PE66 KDEL had potent cytotoxicity on the target cells with high affinity IL-2R through the specific binding of IL-2 moiety to IL-2R and the toxicity of the PE moiety. The results showed that the sensitivity of the same target cells to various IL2-PE fusion proteins were different ( for example , the cytotoxicity of IL2-PE66 KDEL on the PHA-activated blasts of human peripheral blood was about 40 times as much as that of IL2-PE40REDLK ) .It was also discovered that the carbonyl terminal REDLK sequence of IL-2-PE fusion proteins substituted by KDEL would enhance their cytotoxicity ( for example , the cytotoxicity of IL2-PE40 KDEL on .the ConA-stimulated murine spleen blasts was about 8 times than the one of IL2-PE40REDLK ) . |
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| Keywords: | Interleukin-2 Pseudomonas exotoxin Fusion protein Cytotoxicity Sitedirected mutagenesis |
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