| RNA干扰HESR1基因质粒体的构建 |
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| 引用本文： | 李斌,唐仕波,林少芬,孟晶,肖迎. RNA干扰HESR1基因质粒体的构建[J]. 眼科学报, 2005, 21(2): 112-115 |
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| 作者姓名： | 李斌 唐仕波 林少芬 孟晶 肖迎 |
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| 作者单位： | 中山大学中山眼科中心,广州,510060 |
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| 摘 要： | 目的:构建HESR1基因的RNA干扰(RNAi)载体。方法:根据人HESR1的基因序列设计短双链两条,人工合成后,连接到RNAi-ReadypSIREN-RetroQZsGreenVector载体中,构成两个HESR1/RNAi重组质粒,转入内皮细胞,逆转录聚合酶链反应(RT-PCR)检测两质粒对HESR1表达的影响,以确定HESR1/RNAi重组质粒进行下一步研究。结果:两质粒都有沉默HESR1基因的作用,1号作用更强烈一些,确定使用1号pSIREN/HESR1质粒进行下一步研究。结论:HESR1/RNAi重组质粒的成功构建,为下一步研究奠定基础。
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| 关 键 词： | HESR1基因 RNA干扰 载体 |
Construction of HESR1 siRNA Plasmid Expression Vector |
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| Bin Li,Shibo Tang,Shaofen Lin,Jing Meng,YING XIAO. Construction of HESR1 siRNA Plasmid Expression Vector[J]. Eye science, 2005, 21(2): 112-115 |
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| Authors: | Bin Li Shibo Tang Shaofen Lin Jing Meng YING XIAO |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University Guangzhou 510060, China. |
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| Abstract: | Purpose:To construct of HESR1 siRNA plasmid expression vector. Methods:Cultures human umbilicus vein endothelial cell, accoding to the computer aided design, HESR1-specific siRNA gene was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed HESR1-RNAi plasmid were transfected into human umbilicus vein endothelial cell, RT-PCR detected the effected of HESR1-RNAi plasmid. Result:The recombinant was cloned and the sequence was obtained, HESR1-RNAi plasmid can down-regulated the expression of HESR1 in human umbilicus vein endothelial cell. Conclution:Susccessfully cloning the recombinant make it possible searching new mechanism of HESR1 in angiogenesis |
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| Keywords: | HESR1 gene RNAi vector |
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