当归-白芍药对治疗溃疡性结肠炎的网络药理学研究

目的基于网络药理学研究当归-白芍药对治疗溃疡性结肠炎的作用机制。方法利用TCMSP数据库,得到当归-白芍药对化学成分的口服利用率和药物相似性等参数,并筛选药对的有效成分组。利用UniProt数据库找到当归-白芍药对有效靶点的官方基因名称,并利用CTD数据库进行靶点相关疾病注释。通过DisGeNET数据库得到溃疡性结肠炎的相关靶点,并与当归-白芍作用靶点取交集,得到其治疗溃疡性结肠炎的药效靶点。将上述靶点输入到String数据库进行蛋白质-蛋白质相互作用网络预测,最后利用DAVID数据库进行KEGG富集分析,揭示当归-白芍药对治疗溃疡性结肠炎的潜在信号通路。结果筛选出了当归的2个活性成分和白芍的9个活性成分以及63个抗溃疡性结肠炎的靶点。通过蛋白质互作网络分析,当归-白芍药对治疗溃疡性结肠炎可能与MAPK14、PTSG2、PPARG、TNF、IL6、NOS3、BCL2等蛋白质有关。此外,当归-白芍药对治疗溃疡性结肠炎的靶点显著富集于HIF-1、TNF、NF-κB等信号通路中。结论当归-白芍药对具有多靶点、多途径、多通路的特点,其治疗溃疡性结肠炎的潜在生物学机制可能与以HIF-1、TNF、NF-κB等信号通路有关。

Network Pharmacology Study of Angelica Sinensis-Radix Paeoniae Albain Treatment of Ulcerative Colitis

Objective To study the mechanism of Angelica sinensis-Radix Paeoniae Alba in the treatment of ulcerative colitis based on network pharmacology. Methods Using the TCMSP database, the oral utilization rate and drug similarity of the chemical constituents of Angelica sinensis-Radix Paeoniae Alba were obtained, and the effective component groups of the drug pairs were screened. The UniProt database was used to find out the official gene name for the effective targetand the CTD database was used for annotation of the target-related disease. The target of ulcerative colitis was obtained through DisGeNET database, and it was combined with the target of Angelica sinensis to obtain its pharmacological target for treating ulcerative colitis. The above target was input into the String database for protein-protein interaction network prediction. Finally, the KEGG enrichment analysis was performed using the DAVID database to reveal the potential signaling pathway of Angelica sinensis-Radix Paeoniae Albato treat ulcerative colitis. Results We screened two active ingredients of Angelica and nine active ingredients ofRadix Paeoniae Alba and 63 targets for ulcerative colitis. Through protein interaction network analysis, Angelica sinensis-Radix Paeoniae Albacan be associated with MAPK14, PTSG2, PPARG, TNF, IL6, NOS3, BCL2 and other proteins in the treatment of ulcerative colitis. In addition, the target of Angelica sinensis-Radix Paeoniae Albafor the treatment of ulcerative colitis is significantly enriched in the signaling pathways,such as HIF-1 signaling pathway, TNF signaling pathwayand NF-κB signaling pathway. Conclusion Angelica sinensis-Radix Paeoniae Albahas the characteristics of multiple targets, multiple pathways and multiple signals. The potential biological mechanism of the treatment of ulcerative colitis may be related to HIF-1 signaling pathway, TNF signaling pathway and NF-κB signaling pathway. It is related to the signal path.