姜黄素抑制TLR4/HMGB1通路保护脂多糖诱导急性肺损伤的作用

目的探讨姜黄素对脂多糖(LPS)诱导急性肺损伤的保护作用及相关机制。方法将24只SD大鼠随机分为对照组、LPS组和LPS+姜黄素组,每组8只。检测肺损伤指标(氧分压、肺干湿重比、肺病理评分)、肺组织炎症反应程度肿瘤坏死因子(TNF)-α、白介素(IL)-6、单核细胞趋化蛋白(MCP)-1以及肺组织中Toll样受体4(TLR4)、高迁移率族蛋白B1(HMGB1)表达水平]。结果LPS组氧分压显著低于对照组(P<0.05),而肺干湿重比、肺病理评分、TNF-α、IL-6、MCP-1、TLR4、HMGB1表达水平显著高于对照组(P<0.05);LPS+姜黄素组氧分压高于LPS组(P<0.05),肺干湿重比、肺病理评分、TNF-α、IL-6、MCP-1、TLR4、HMGB1表达水平显著低于LPS组(P<0.05)。结论姜黄素可能通过抑制TLR4/HMGB1通路,降低肺炎症反应程度,发挥保护LPS诱导急性肺损伤作用。

Curcumin inhibits Toll-like receptor 4/high mobility group box 1 pathway to protect lipopolysaccharide-induced acute lung injury

Objective To investigate the protective effect and mechanism of curcumin on lipopolysaccharide(LPS)-induced acute lung injury.Methods Totally 24 SD rats were randomly divided into a control group,a LPS group and a LPS+curcumin group(n=8 in each group).The degree of lung injury(oxygen partial pressure,wet/dry ratio,pathological scores)and inflammatory levelstumor necrosis factor(TNF)-α,interleukin(IL)-6,monocyte chemotactic protein(MCP)-1,Toll-like receptor 4(TLR4),mobility group box 1 protein(HMGB1)expression]of the lung were detected in different groups.Results Oxygen partial pressure was significantly lower in the LPS group than that in the control group(P<0.05),while wet/dry ratio,pathological scores and expression levels of TNF-α,IL-6,MCP-1,TLR4 and HMGB1 were significantly higher in the LPS group than those in the control group(P<0.05).Compared with the LPS group,curcumin significantly reduced wet/dry ratio,pathological scores and expression levels of TNF-α,IL-6,MCP-1,TLR4 and HMGB1 in the LPS+curcumin group(P<0.05),while it significantly improved oxygen partial pressure(P<0.05).Conclusion Curcumin might protect LPS-induced acute lung injury through inhibition of TLR4-HMGB1-inflammation pathway.