基于网络药理学分析莪术的作用机制

目的运用网络药理学的方法研究莪术药理作用的物质基础和作用机制。方法采用中药系统药理学分析平台(TCMSP)搜索莪术的化学成分、作用靶点、治疗疾病。从而建立化合物-靶点网络、蛋白质相互作用(PPI)网络、靶点-疾病网络、基因-通路网络、基因本体(GO)功能和通路富集分析,研究莪术作用机制。结果筛选得到6个化合物,36个靶点(其中两个化合物没有相应的靶点)相对应疾病102种。PPI网络中有32个靶点,关键靶点有ESR1、AR、NR3C1等。基因功能富集分析共涉及13个条目,其中细胞组成5个,分子功能6个,生物过程2个。通路富集分析得到三条通路,包括神经活性配体-受体相互作用信号通路、逆行内源性大麻素信号通路、烟瘾信号通路。结论本研究初步验证莪术药理作用的分子机制,为进一步深入研究其作用机制提供理论基础。

Mechanism of Curcuma Based on Network Pharmacology

Objective To study the material basis and mechanism of pharmacological action of curcuma with the method of network pharmacology.Methods This study used the system pharmacology analysis platform of Chinese medicine to search the chemical components of curcuma,the target point and the treatment of disease.Therefore,the compound target network,the protein interaction(PPI)network and the target disease were established.Disease network,gene pathway network,gene noumenon(go)function and pathway enrichment analysis were used to study the mechanism of curcuma action.Results Six compounds were screened and 36 targets(two of them without corresponding targets)had 32 targets in 102.PPI networks with relative disease,and the key targets were ESR1,AR,NR3 C1 and so on.Thirteen items were involved,including 5 cells,6 molecular functions and 2 biological processes.Three pathways were obtained by pathway enrichment analysis,including Neuroactive ligand-receptor interaction signal pathway,Retrograde endocannabinoid signaling pathway and Nicotine addiction signal pathway.Conclusion This study preliminarily verified the molecular mechanism of curcuma pharmacological action.It provides a theoretical basis for further study of its mechanism.