| PML重组腺病毒载体的构建与鉴定 |
| |
| 引用本文： | 刘宾娜,张士德. PML重组腺病毒载体的构建与鉴定[J]. 牡丹江医学院学报, 2007, 28(2): 16-19 |
| |
| 作者姓名： | 刘宾娜 张士德 |
| |
| 作者单位： | 牡丹江医学院临床系办公室,157011;哈尔滨医科大学第二临床医学院介入科 |
| |
| 摘 要： | 目的：构建携带PML目的基因的重组腺病毒载体（Ad-pml）。方法：应用基因重组技术将PSG-CMV和PSG5-PML分别用EcoRI和BglII双酶切,构建PSG-PML载体。将质粒PSG-PML与含有5型腺病毒右臂的质粒pPE3共转染至293细胞,产生含PML基因的重组腺病毒（Ad-pml）,并经PCR及DNA测序鉴定。结果：经PCR反应可扩增出1767bp大小的片段,测序结果检测Ad-pml序列正确,通过免疫荧光反应证明病毒包装成功并且具有感染力。结论：成功构建了重组腺病毒载体（Ad-pml）,为研究肿瘤基因治疗提供了基础。
|
| 关 键 词： | PML基因 腺病毒载体 重组 |
| 文章编号： | 24196577 |
| 修稿时间： | 2006-12-29 |
CONSTRUCTION AND IDENTIFICATION OF RECOMBINANT ADENOVIRUS CONTAINING HUMANPML GENE |
| |
| Liu binna et al. CONSTRUCTION AND IDENTIFICATION OF RECOMBINANT ADENOVIRUS CONTAINING HUMANPML GENE[J]. Journal of Mudanjiang Medical College, 2007, 28(2): 16-19 |
| |
| Authors: | Liu binna et al |
| |
| Affiliation: | Clinical depantment of MuDanjiang Medical college 157011 |
| |
| Abstract: | Objective:To construct the recombinant adenovirus plasmid carring human PML gene for further study.Methods:By gene recombinantion technique, the plasmid of PSG-CMV and PSG5-PML, double-digested with restrictive endonucleases EcoRI and BglII, constructed PSG-PML vector. Finally,the PSG-PML recombinant plasmid was transfected into 293 cell with pPE3 containing Adenovirus type 5 that including Right Arm ,which produced Ad-pml. Ad-pml was verified by PCR and DNA sequence.Results:Identification of the Ad-pml by restriction digestion and PCR showed the length. Ad-pml was confirmed in sequence.fluorescence showed successful recombined adenovirus with infectiveness.Conclusion:Ad-pml was successfully constructed.The development of Ad-pml will provide the basis for the research and therapy of cancer. |
| |
| Keywords: | PML gene Adenovirus vector Recombination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
