| 20-羟基蜕皮甾酮对H_2O_2诱导SH-SY5Y细胞损伤的保护作用 |
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| 引用本文： | 司霞,马卓,陈月,黄琳,冯婉玉.20-羟基蜕皮甾酮对H_2O_2诱导SH-SY5Y细胞损伤的保护作用[J].中国药学,2014,23(1):33-38. |
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| 作者姓名： | 司霞 马卓 陈月 黄琳 冯婉玉 |
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| 作者单位： | [1]北京大学人民医院药剂科,北京100044 [2]北京大学医学部药学院药事管理与临床药学系,北京100191 |
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| 基金项目： | We thank Zhanyou Wang for generously providing the human neuroblastoma SH-SY5Y cells. We are also grateful to Mei Li and Xing Yu for technical assistance. |
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| 摘 要： | 本文旨在研究一种类固醇激素即20-羟基蜕皮甾酮对H2O2诱导的SH-SY5Y细胞损伤的保护作用及其作用机制。预孵育20-羟基蜕皮甾酮可以明显提高H2O2诱导的SH-SY5Y细胞的生存率,减少乳酸脱氢酶（LDH）漏出率,抑制脂质过氧化水平（MDA）升高,提高超氧化物歧化酶（SOD）活性。此外,20-羟基蜕皮甾酮可以通过降低Bax/Bcl-2的比值,延缓caspase-3的活化来抑制H2O2诱导的细胞凋亡。本研究表明20-羟基蜕皮甾酮对H2O2诱导的SH-SY5Y细胞具有保护作用,有可能用于由氧化应激和凋亡导致的神经退行性疾病的治疗。
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| 关 键 词： | 20-羟基蜕皮甾酮 双氧水 SH-SY5Y细胞 氧化应激 凋亡 神经退行性疾病 |
Protective effects of 20-hydroxyecdysone on H2O2-induced cytotoxicity in human neuroblastoma SH-SY5Y cells |
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| Xia Si,Zhuo Ma,Yue Chen,Lin Huang,Wanyu Feng.Protective effects of 20-hydroxyecdysone on H2O2-induced cytotoxicity in human neuroblastoma SH-SY5Y cells[J].Journal of Chinese Pharmaceutical Sciences,2014,23(1):33-38. |
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| Authors: | Xia Si Zhuo Ma Yue Chen Lin Huang Wanyu Feng |
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| Institution: | 1. Department of Pharmaey, Peking University People＇s Hospital, Beij＇ing 100044, China 2. Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University Health Science Center, Beij ing 100191, China) |
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| Abstract: | The aim of this study was to investigate the possible protective effects and mechanisms of 20-hydroxyecdysone, an insect steroid hormone, on HEO2-induced cytotoxicity in human neuroblastoma SH-SYSY cells. Pretreatment with 20-hydroxyecdysone significantly elevated the cell viability and decreased LDH leakage in H2O2-treated SH-SY5Y cells. 20-Hydroxyecdysone also dramatically reduced malondialdehyde （MDA） contents and enhanced the superoxide dismutase （SOD） activities under oxidative stress conditions. Furthermore, 20-hydroxyecdysone pretreatment inhibited apoptosis by decreasing the Bax/Bcl-2 ratio and attenuating the activation of caspase-3. These results suggest that 20-hydroxyecdysone can protect SH-SYSY cells against H2O2-induced cytotoxicity and might potentially be used to treat neurodegenerative diseases induced by oxidative stress and anontosis. |
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| Keywords: | 20-Hydroxyecdysone H2O2 SH-SY5Y cells Oxidative stress Apoptosis Neurodegenerative disease |
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